目的 研究滇西南地区埃及伊蚊种群遗传学特征，针对该地区的埃及伊蚊种群筛选新的微卫星位点。方法 从美国国立生物技术信息中心GenBank数据库中下载埃及伊蚊全基因组数据，利用Tandem Repeats Finder软件查找全基因组中的微卫星位点，选择重复单位为2~4 bp且重复次数< 30的微卫星位点，对其侧链进行BLAST比对，选择在基因组中单拷贝的位点设计引物，再对引物进行BLAST比对，选择扩增产物为单拷贝的引物进行相关筛选。结果 从17个全基因组中共获得80条微卫星序列，并成功设计出11对多态性引物，核心序列包括5对2碱基重复，5对3碱基重复以及1对4碱基重复，共有98个等位基因，平均每对引物有9个等位基因，观察杂合度平均值为（0.394±0.026）。结论 设计引物可稳定地扩增出目标条带，且多态性较高，可以用于埃及伊蚊的种群遗传学研究。
Objective To obtain useful microsatellite loci of Aedes aegypti from the southwest areas of Yunnan province. Methods The Ae. aegypti genome was downloaded from National Center for Biotechnology Information(NCBI). The repeats with a period size of 2-4 bp and a copy number less than 30 were chosen using Tandem Repeats Finder software. Primers were designed with those microsatellites of unique flanking, and BLAST was utilized for these primer sequences to confirm amplicons also single copy. Results A total of 80 microsatellite loci were obtained from 17 whole genomes and 11 pairs of primer were designed successfully with 98 alleles. There are 9 alleles per pair of primers on average. The average value of observation heterozygosity is(0.394±0.026). Conclusion The acquired primers can amplify bands stably and these amplicons are polymorphic, being fit for the research of population genetics of Ae. aegypti.
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