目的 了解贵阳市致倦库蚊和白纹伊蚊的抗药性现状，为制定蚊虫防制措施提供依据。方法 2015年8-9月采集贵阳市8个区（县）的野外致倦库蚊和白纹伊蚊幼虫，在实验室饲养后，采用幼虫浸渍法测定其对4种常用杀虫剂的抗药性。结果 致倦库蚊幼虫对DDVP、双硫磷、高效氯氰菊酯和溴氰菊酯的抗性系数分别为5.07~10.79、8.16~10.20、13.92~27.22和100.00~226.32倍；白纹伊蚊幼虫对4种杀虫剂的抗性系数依次分别为7.61~25.69、4.76~28.57、6.42~76.19和14.75~59.02倍。结论 贵阳市致倦库蚊和白纹伊蚊对4种常用杀虫剂均产生了一定程度的抗药性，其中对拟除虫菊酯类杀虫剂的抗药性较高。建议今后灭蚊应减少或避免长期、单一、大量使用此类杀虫剂，同时需加强蚊虫抗药性监测，并采用综合措施。

Objective To understand the insecticide resistance status of Culex pipiens quinquefasciatus and Aedes albopictus in Guiyang, China, to provide scientific basis in rational use of chemical insecticides for management of mosquito-borne diseases. Methods Larval mosquitoes of Cx. pipiens quinquefasciatus and Ae. albopictus were collected in 5 districts and 3 counties of Guiyang, and were reared in the insectary. The larvae dipping method was used to detect the insecticide resistance to the four insecticides. Results The resistance ratios of Cx. pipiens quinquefasciatus larvae to dichlorvos, parathion, beta-cypermethrin, and deltamethrin were 5.07-10.79, 8.16-10.20, 13.92-27.22, and 100.00-226.32 fold. The resistance ratios of Ae. albopictus larvae to the four insecticides were 7.61-25.69, 4.76-28.57, 6.42-76.19, and 14.75-59.02 fold. Conclusion Culex pipiens quinquefasciatus and Ae. albopictus larvae in Guiyang have developed certain resistance, in which the mosquito had a higher resistance to pyrethroid insecticides. We suggest reducing or avoiding pyrethroid insecticides for a single, extensive and long-term use, and to strengthen the insecticide resistance surveillance for better mosquito prevention and control.