目的 通过准确地鉴定中山口岸入境船舶截获的蜚蠊,为今后口岸鉴定该种蜚蠊提供参考依据。方法 2017年2月24日在中山口岸截获的圭亚那进口原木中截获1只雄性蜚蠊,对其进行形态鉴定及描述,获取其DNA条形码序列并提交至GenBank。结果 经形态鉴定,该雄性蜚蠊为染色硬翅蠊,隶属于蜚蠊目姬蠊科。经科技文献查新,确认为我国未见分布种且为国内口岸首次截获。结论 DNA条形码是形态鉴定的强有力补充,DNA条形码数据库有待完善和补充,应用形态鉴定和DNA条形码相结合可准确快速地鉴定外来蜚蠊。
Objective To identify the intercepted cockroach rapidly and accurately. Methods We tried to identify the male cockroach intercepted from an entry ship at Zhongshan port on February 24, 2017 with morphology, then DNA barcodes. DNA barcodes was submitted to the GenBank and the morphological characteristics of the intercepted sample were illustrated as well. Results The male individual was identified as Ceratinoptera picta, belonging to Ectobiidae, Blattodea. Ceratinoptera picta is a non-recorded species in China and it is intercepted for the first time at Chinese ports based on Sci-Tech novelty search. Conclusion DNA barcoding is a strong supplement to morphology. At present, DNA Barcoding Data system needs to be replenished and provides references for the future use. Combining morphology and DNA barcoding techniques is suggested in identification of intercepted cockroach rapidly and accurately.
[1] 廖如燕,张显光,谢勇秋,等. 一艘入境巨轮上60余万只蜚蠊的卫生处理[J]. 中国国境卫生检疫杂志,2017,40(1):73-74.
[2] 张治富,宁瑞瑜,高建功,等. 国际航行船舶外来蜚蠊传入风险的logistic回归分析[J]. 中国国境卫生检疫杂志,2014,37(3):203-206.
[3] 田洁,郭惠琳,黄健华,等. 境外宠物蜚蠊传入风险及控制[J]. 中国媒介生物学及控制杂志,2013,24(6):558-560.
[4] 岳巧云,邱德义,胡佳,等. DNA条形码:医学媒介生物快速准确鉴定的利器[J]. 检验检疫学刊,2013,23(5):60-63,49.
[5] Hebert PDN,Cywinska A,Ball SL,et al. Biological identifications through DNA barcodes[J]. Proc Biol Sci,2003,270(1512):313-321.
[6] 魏晓雅,邱德义,岳巧云,等. 口岸截获国内未见分布蜚蠊:斯科特卡蠊的形态和DNA条形码鉴定[J]. 中国国境卫生检疫杂志,2016,39(6):381-384,449.
[7] 廖俊蕾,岳巧云,邱德义,等. 新截获中国未见分布种杜比亚丽蝇形态和DNA条形码鉴定[J]. 中国媒介生物学及控制杂志,2014,25(6):509-513.
[8] 单振菊,刘德星,岳巧云,等. 口岸截获国内未见分布种褐小家鼠的DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2016,27(1):17-20.
[9] 江莉,龚劲聪,胡龙飞,等. DNA条形码技术用于进口货物中肩突硬蜱的鉴定[J]. 中国国境卫生检疫杂志,2014,37(1):33-36.
[10] Bergsten J, Brilmyer G, Crampton-Platt A, et al. Sympatry and colour variation disguised well-differentiated sister species:Suphrodytes revised with integrative taxonomy including 5 kbp of housekeeping genes (Coleoptera:Dytiscidae)[J]. DNA Barcodes,2012,1(1):1-18.
[11] Von Wattenwyl KB. Nouveau système des Blattaires[M]. Vienna:G. Braumüller,1865:76-77.
[12] Bruner L. Report on the Orthoptera of Trinidad, West Indies[J]. J New York Entomol Soc,1906,14(3):135-165.
[13] Hebard M. The genus Ceratinoptera (Orthoptera, Blattidae, Pseudomopinae)[J]. Trans Am Entomol Soc,1916,42(2):125-134.
[14] Fisk FW, Wolda H. New species of Ceratinoptera cockroaches from Panama and Costa Rica (Blattaria:Blattellidae:Plectopterinae)[J]. Proc Entomol Soc Wash,1983,85(2):286-296.
[15] Folmer O, Black M, Hoeh W, et al. DNA primers for amplification of mitochondrial cytochrome C oxidase subunit I from diverse metazoan invertebrates[J]. Mol Mar Biol Biotechnol,1994,3(5):294-299.
[16] 刘德星,岳巧云,廖俊蕾,等. 国内未见分布蝇种:澳洲麻蝇的形态和DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2015,26(3):282-285.
[17] Renaud AK, Savage J, Adamowicz SJ. DNA barcoding of northern Nearctic Muscidae (Diptera) reveals high correspondence between morphological and molecular species limits[J]. BMC Ecol,2012,12:24. DOI:10.1186/1472-6785-12-24.
[18] Rivera J, Currie DC. Identification of Nearctic black flies using DNA barcodes (Diptera:Simuliidae)[J]. Mol Ecol Resour, 2009,9 Suppl 1:224-236.
[19] Schuehli GSE, de Carvalho CJB, Wiegmann BM. Molecular phylogenetics of the Muscidae (Diptera:Calyptratae):new ideas in a congruence context[J]. Invertebr Syst,2007,21(3):263-278.
[20] 王宗庆,车艳丽. 世界蜚蠊系统学研究进展(蜚蠊目)[J]. 昆虫分类学报,2010,32增刊:23-33.