目的 分析我国室内15种常见蜚蠊的细胞色素C氧化酶亚基Ⅰ（COⅠ）基因序列，为建立蜚蠊分子鉴定方法奠定基础。方法 通过美国国立生物技术信息中心检索我国室内常见蜚蠊线粒体COⅠ基因序列，应用DNAStar和MEGA 6.0软件进行序列分析，探讨种间差异和遗传关系，利用Neighbor-Joining法构建系统进化树。结果 我国室内常见15种蜚蠊种平均进化分歧数为0.166，种间差异较大；系统进化树分析显示，同属蜚蠊COⅠ分子标记分类结果与传统形态分类学基本一致，且可信度均>91。结论 基于658 bp COⅠ基因序列，可对我国室内部分蜚蠊进行种下水平的分类鉴定，而该基因对于属或科以上水平的区分及少数近缘种的鉴定有所限制。
Objective To provide basis for future molecular identification methods of cockroach species by analyzing gene sequences of cytochrome C oxidase subunitⅠ(COⅠ) among 15 cockroach species. Methods The mitochondrial COⅠ gene sequences of cockroaches were searched by NCBI and the sequence alignment was carried out by DNAStar software. An evolutionary divergence and a Neighbor-Joining tree were constructed using MEGA 6.0 software. Results The average interspecific genetic distances among 15 species of cockroaches in China was 0.166, and the genetic distances among species were significant. Phylogenetic tree analysis showed that COⅠ molecular markers were the same as traditional morphological classification, and the confidentiality more than 91. Conclusion The COⅠ gene can be effectively used to distinguish some species of domestic cockroaches. The value of 658 bp region of the mitochondrial COⅠ gene for identification of genus or subspecies is limited.
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