目的 对山西省吕梁市方山县南阳沟林场进行莱姆病传播媒介调查，明确是否存在莱姆病自然疫源地。方法 2015年采用布旗法在方山县南阳沟林场捕蜱，鉴定后采用BSKⅡ培养基对其进行分离培养；采用多位点序列分析（MLSA）法对分离菌株进行分型鉴定。结果 共采蜱约300只，包括全沟硬蜱、嗜群血蜱和边缘革蜱，其中全沟硬蜱为当地优势蜱种。从全沟硬蜱和嗜群血蜱中分离出2株伯氏疏螺旋体菌株，经MLSA鉴定为Borrelia garinii基因型。结论 山西省吕梁市方山县南阳沟林场为莱姆病自然疫源地。应加强对当地人群、媒介及宿主的调查，为防控莱姆病提供科学依据。
Objective Investigateon on vectors was carried out to identify the natural foci of Lyme disease in Nanyanggou forest area of Fangshan county of Lyuliang, Shanxi province. Methods Flagging and trapping methods were used to collect ticks, BSKⅡ media was used to isolate Lyme spirochetes from ticks. MLSA method was used to genotype the isolates. Results About 300 ticks were collected from Nanyanggou forest area, including Ixodes persulcatus, Haemaphysalis concinna, and Dermacentorm marginatus. Ixodes persulcatus was the predorminant species, which was accounted for 80% among the ticks. Two strains were isolated from I. persulcatus and H. concinna, respectively. MLSA analysis showed that 2 strains isolated from ticks belonged to Borrelia garinii. Conclusion There exists natural foci of Lyme disease in Lyuliang, Shanxi province. Detailed survey on people, vector and rodents should be carried out to provide data for prevention and control of Lyme disease in this area.
[1] Stanek G, Wormser GP, Gray J, et al. Lyme borreliosis[J]. Lancet, 2012, 379(9814):461-473.
[2] Margos G,Vollmer SA,Ogden NH,et al. Population genetics, taxonomy,phylogeny and evolution of Borrelia burgdorferi sensu lato[J]. Infect Genet Evol, 2011, 11(7):1545-1563.
[3] Hao Q, Hou XX, Geng Z, et al. Distribution of Borrelia burgdorferi sensu lato in China[J]. J Clin Microbiol, 2011, 49(2):647-650.
[4] 张哲夫,万康林,张金声,等. 我国莱姆病的流行病学和病原学研究[J]. 中华流行病学杂志, 1997, 18(1):8-11.
[5] 耿震,侯学霞,万康林,等. 中国6省蜱中莱姆病螺旋体分离与鉴定[J]. 中华流行病学杂志, 2010, 31(12):1346-1348.
[6] 侯学霞,耿震,郝琴,等. 中国6省莱姆病螺旋体主要宿主动物鼠的初步调查[J]. 中国人兽共患病学报, 2010, 26(11):1034-1036.
[7] 耿震,李国华,侯学霞,等. 山西省首例莱姆病病例分析[J]. 中国媒介生物学及控制杂志, 2014, 25(4):318-319, 322.
[8] 张杰敏,杨慧军,李效民,等. 山西省某县莱姆病感染情况调查[J]. 中国药物与临床, 2006, 6(7):490-491.
[9] 刘明社,武延隽,赵中夫. 山西长治地区就诊人群莱姆病血清学初步调查[J]. 长治医学院学报, 2006, 20(1):17-18.
[10] 陆宝麟,吴厚永. 中国重要医学昆虫分类与鉴别[M]. 郑州:河南科学技术出版社, 2003:652-679.
[11] Rudenko N,Golovchenko M,Grubhoffer L,et al. Updates on Borrelia burgdorferi sensu lato complex with respect to public health[J]. Ticks Tick Borne Dis, 2011, 2(3):123-128.
