目的 探索黑龙江省同江口岸鼠类中可能携带Bartonella washoensis的分子生物学证据。方法 2016年6月，在黑龙江省同江口岸林缘地中，采用夹捕法和笼捕法捕获啮齿动物，采集啮齿动物脾样本，利用巴尔通体rpoB基因序列保守区引物，用PCR法检测巴尔通体，获得的阳性样本通过基因测序进一步分析。结果 在捕获的113只鼠中共获得69份阳性样本，获得的序列与GenBank中的序列进行比对，在花鼠脾脏中获得的巴尔通体rpoB基因片段（编号：TJ2016-107）与同样是分离自花鼠的B. washoensis SR22-1株和SR25-1株同源性最高，分别为99%和98%。提示黑龙江省同江口岸花鼠中可能携带B. washoensis。结论 首次在花鼠脾脏中扩增出与B. washoensis同源性较高的rpoB基因片段，这也是黑龙江省可能存在B. washoensis的直接证据。
石金峰, 耿聪, 张坤, 鞠文东, 王力翔, 王红霞, 李振江, 黄晓明, 程成
. 黑龙江省同江口岸首次发现花鼠携带Bartonella washoensis的分子生物学证据[J]. 中国媒介生物学及控制杂志, 2017
, 28(5)
: 484
-486
.
DOI: 10.11853/j.issn.1003.8280.2017.05.020
Objective To explore the molecular biological evidence that rodents may carry Bartonella washoensis in Heilongjiang Tongjiang port. Methods Rodents were captured by night snaptrap and cagetrap method in June 2016. Spleen samples were aseptically collected to detect Bartonella by PCR of which primers were designed using the conserved region of the rpoB gene sequence of Bartonella spp. The positive samples obtained were further identified through gene sequencing. Results The results showed that 69 positive samples in all rodents, sequence alignment in GenBank showed that Bartonella rpoB gene fragment(No. TJ2016-107)obtained from Tamias sibiricus had high homology with SR22-1 and SR25-1 strain of B. washoensis, 99% and 98%. Conclusion It is the first time that the Bartonella rpoB gene positive fragments were amplified in the spleen of T. sibiricus, which is a high homology with B. washoensis. It is also the direct evidence that B. washoensis may exist in Heilongjiang.
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