目的 了解杭州市家蝇对5种常见卫生杀虫剂的抗药性，并与近20年的监测结果进行比对，探索杭州市家蝇抗药性变迁规律，为科学防控提供依据。方法 2014年6-7月，采集杭州市建德市、江干区、下城区等不同方位的家蝇，采用微量点滴法测定现场种群成蝇对5种常用卫生杀虫剂的抗药性。结果 现场种群家蝇对残杀威抗性水平最高，抗性倍数>1345倍，对溴氰菊酯、高效氯氰菊酯、DDVP和三氯杀虫酯的抗性倍数依次为77.33（LD₅₀为0.0696μg/只）、55.58（LD₅₀为0.2001μg/只）、7.26（LD₅₀为0.3290μg/只）和6.79（LD₅₀为1.4608μg/只）倍。历年监测资料显示，溴氰菊酯、高效氯氰菊酯一直处于高抗水平；DDVP抗性也呈现逐年升高趋势，2008年达到最高，之后又有所下降；残杀威抗性急剧上升；三氯杀虫酯抗性有一定上升趋势。结论 杭州市家蝇现场种群已对多种杀虫药剂产生抗性，部分药剂如残杀威已基本无效。杭州市蝇类控制工作应进一步强化可持续控制策略，以环境治理与物理防治为主要控制手段，必要时选择抗性低的化学杀虫剂，科学施药，以减缓或防止家蝇的抗性产生。

Objective To investigate the resistance of Musca domestica to 5 commonly used insecticides in Hangzhou, and to compare the results with those of the last 20 years, providing scientific basis for prevention and control. Methods Musca domestica were collected from Jiande city, Jianggan district and Xiacheng district of Hangzhou city from June to July, 2014. Topical application was used to determine the resistance of M. domestica to 5 commonly used insecticides. Results The resistance to propoxur was the highest, the resistances ratios (R/R)>1 345. The resistances ratios to deltamethrin, beta-cypermethrin, dichlorvos, and three chlorine insecticidal ester was 77.33(LD₅₀=0.069 6 μg/♀), 55.58 (LD₅₀=0.200 1 μg/♀), 7.26(LD₅₀=0.329 0 μg/♀), and 6.79(LD₅₀=1.460 8 μg/♀), respectively. The monitoring data showed that the resistance to deltamethrin and beta-cypermethrin were at a high level; the resistance to dichlorvos showed an increasing trend year by year, the highest in 2008, and then declined; the resistance to propoxur increased sharply, the resistance to three chlorine insecticidal ester showed a rising trend. Conclusion The M. domestica strains of Hangzhou have been resistant to many kinds of insecticides. Some insecticides, such as propoxur, have been ineffective. Sustainable integrated response strategies should be established in M. domestica control in Hangzhou. In order to slow down or prevent resistance, environmental control and physical control should be considered as the main control means; if necessary, chemical insecticides with low resistance can be selected based on the resistance monitoring data.