目的 了解云南省耿马县埃及伊蚊对常用卫生杀虫剂的抗药性水平。方法 以云南省临沧市耿马县孟定镇为调查点,2016年9月采集埃及伊蚊幼虫并饲养,采用成蚊滤纸接触筒法,测定其对6种常用卫生杀虫剂的敏感性,根据校正死亡率判定抗性级别。结果 耿马县孟定镇埃及伊蚊对氟氯氰菊酯、氯菊酯、顺式氯氰菊酯、杀螟硫磷、马拉硫磷、残杀威1 h的击倒率分别为10.09%、24.52%、100%、90.72%、100%和100%,24 h死亡率分别为10.09%、41.94%、64.17%、100%、100%和100%;半数击倒时间(KT50)分别为9.70、8.59、0.47、5.45、3.55和0.55 min。孟定镇埃及伊蚊对顺式氯氰菊酯、氯菊酯、氟氯氰菊酯均呈抗性水平,对杀螟硫磷、马拉硫磷、残杀威杀虫剂均敏感。结论 云南省耿马县埃及伊蚊对拟除虫菊酯类杀虫剂均产生了不同程度的抗性,对该地区埃及伊蚊应采取科学合理的抗性管理措施,因地制宜,交替或混合施用不同类型的杀虫剂。
Objective To investigate the resistance level of Aedes aegypti to six commonly used insecticides in Gengma county, Yunnan province. Methods Pupale or eggs of Ae. aegypti were collected in Gengma county of Lincang city and were reared in laboratory to the first generation. The susceptibility of Ae. aegypti to six insecticides were tested by bioassay method. The resistance level was determined by adjusted mortality. Results Aedes aegypti were exposed to 0.03% cyfluthrin, 0.25% permethrin, 0.8% alpha cypermethrin, 0.25% fenitrothion, 0.8% malathion, 0.1% propoxur for 1 h, the knockdown rate was 10.09%, 24.52%, 100%, 90.72%, 100%, and 100%. The mortality after 24 h was 10.09%, 41.94%, 64.17%, 100%, 100%, and 100%. The KT50 was 9.70, 8.59, 0.47, 5.45, 3.55, and 0.55 accordingly. The test subjects were resistant to cyfluthrin, permethrin, alpha cypermethrin but remained susceptible to fenitrothion, malathion, and propoxur. Conclusion Aedes aegypti in Gengma county, Yunnan province showed concurrent resistant to cyfluthrin, permethrin and alpha cypermethrin. Therefore, the control strategy against Ae. aegypti by insecticides should be reevaluated accordingly in the study area.
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