目的 掌握广西壮族自治区（广西）口岸小兽自然感染汉坦病毒、立克次体、伯氏疏螺旋体、巴尔通体和钩端螺旋体（钩体）的情况。方法 于2014-2015年在广西8个口岸辖区范围内采用鼠笼法捕获小兽，取其肺、肝和肾脏组织。从小兽肺中提取RNA，利用RT-PCR扩增汉坦病毒核酸；从小兽肝和肾脏中提取DNA，用PCR方法分别扩增立克次体、伯氏疏螺旋体、巴尔通体和钩体核酸。结果 捕获黄胸鼠、褐家鼠和臭鼩鼱共86只，其中褐家鼠数量最多，占捕获总数的67.4%（58/86）；汉坦病毒核酸、巴尔通体和钩体总阳性率分别为3.5%（3/86）、2.3%（2/86）和35.3%（6/17）；未检测到立克次体和伯氏疏螺旋体核酸。结论 广西地区部分口岸小兽可能有汉坦病毒、巴尔通体和钩体自然感染，应加强口岸小兽及其传播疾病的监测。

Objective To understand the prevalence of rodent-borne pathogens in the ports of Guangxi Zhuang Autonomous Region. Methods The small mammals were captured by mouse cages in eight ports in Guangxi. The small mammals lung, liver and kidney specimens were obtained by dissection in laboratory. Total RNA was extracted from lung specimens, and Hantavirus nucleotide acid was amplified by RT-PCR. DNA was extracted from liver and kidney specimens. Rickettsia, Borrelia burgdorferi, Bartonella and Leptospira interrogans nucleotide acid were amplified by PCR, respectively. Results A total of 86 small mammals including Rattus tanezumi, R. norvegicus, and Suncus murinus were captured. The positive rate of Hantavirus, Bartonella, and Leptospira interrogans nucleotide acid were 3.5%(3/86), 2.3%(2/86), and 35.3%(6/17); No Rickettsia and B. burgdorferi spirochetes were detected in this study. Conclusion Hantavirus, Bartonella, and Leptospira interrogans might be carried by small mammals in the regions of Guangxi ports. It is necessary to strengthen rodent-borne diseases surveillance in the ports of Guangxi Zhuang Autonomous Region.