基于DNA条形码丽蝇科49种常见种的分类鉴定

邱德义, 廖俊蕾, 魏晓雅, 岳巧云

doi:10.11853/j.issn.1003.8280.2017.04.005

中国媒介生物学及控制杂志 >

2017 , Vol. 28 >Issue 4: 322 - 331

DOI: https://doi.org/10.11853/j.issn.1003.8280.2017.04.005

论著

基于DNA条形码丽蝇科49种常见种的分类鉴定

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1 中山出入境检验检疫局检验检疫技术中心, 广东中山 528403;
2 中山大学生命科学学院, 广东广州 510275

邱德义,男,高级工程师,从事媒介生物的分子鉴定研究,Email:qiudy@zs.gdciq.gov.cn;廖俊蕾,女,助理研究员,从事媒介生物形态分类和监测研究,Email:904289347@qq.com

收稿日期: 2017-04-17

网络出版日期: 2017-08-20

基金资助

国家质量基础项目（2016YFF0203205）；国家质检总局项目（2015IK067，2015IK069）；广东省国际合作项目（2015A050502009）

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Identification of 49 common fly species in Calliphoridae based on DNA barcodes

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1 Zhongshan Entry-Exit Inspection and Quarantine Technology Center, Zhongshan 528403, Guangdong Province, China;
2 Life College of Sun Yat-sen University

Received date: 2017-04-17

Online published: 2017-08-20

Supported by

Supported by the National Quality Infrastructure Project (No. 2016YFF0203205), Administration of Quality Supervision, Inspection and Quarantine Support Program of China (No. 2015IK067, 2015IK069), and Guangdong Province International Cooperation Project (No. 2015A050502009)

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摘要

目的建立丽蝇科49种常见种的DNA条形码鉴定方法，通过获得其DNA条形码数据为丽蝇科种类鉴定提供技术支持和参比数据，并解决个别雌虫、残缺个体和幼虫等无法准确鉴定的难题。方法对2012-2015年采自全国各地的丽蝇科49种166份标本进行形态学鉴定，并获取相应种类雌雄个体的DNA条形码数据，与已有序列共同构建系统进化树，验证DNA条形码鉴定与形态学鉴定结果的一致性。结果丽蝇科49种的166条序列均提交至GenBank数据库，其中28种丽蝇DNA条形码序列为全球范围内首次获得。丽蝇科同种不同个体间差异<1%，不同种间的DNA条形码差异度随形态学差异的增大而增高。丽蝇科常见种类DNA条形码鉴定结果与形态学鉴定结果一致。结论 DNA条形码是传统分类学的有力补充，特别是针对昆虫的残体、非成虫态及雌性个体均可鉴定，可提高物种鉴定的准确度，对国境口岸准确快速地鉴定媒介生物种类有重要意义。

关键词： 丽蝇科; DNA条形码; 分类鉴定

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邱德义, 廖俊蕾, 魏晓雅, 岳巧云 . 基于DNA条形码丽蝇科49种常见种的分类鉴定[J]. 中国媒介生物学及控制杂志, 2017 , 28(4) : 322 -331 . DOI: 10.11853/j.issn.1003.8280.2017.04.005

Abstract

Objective To establish the DNA barcoding identification techniques for 49 common fly species in Calliphoridae, and provide references data for the identification of these species with DNA barcodes, as to solve the difficulties in the accurate identification of the females, individuals with lost morphological features, and larvae of these species. Methods Totally 166 individuals in Calliphoridae were identified with the morphological characters which were collected from different areas in China during 2012 to 2015, DNA barcodes of the males and females were obtained, an N-J tree was constructed in combination with published data. Consistency of the identification results by morphology and DNA barcodes were tested as well. Results All the 166 sequences of these 49 species were submitted to the public data base, DNA barcodes sequences of 28 species were obtained for the first time. The differences of the intra-species was less than 1%, and the inter-species DNA barcode differences increasing with the morphology differences, and the identification results based on DNA barcodes were consistent with that based on morphology. Conclusion The application of DNA barcodes were strong supplemental to the morphology identification of the species, especially to the incomplete, non-adult and female individuals, and can increase accuracy of identification, and it is very important for the rapid and accurate species identification of the medical vectors at the ports.

Key words： Calliphoridae; DNA barcodes; Species identification

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