目的 调查浙江省职业人群布鲁氏菌病（布病）感染状况及危险因素，为制定科学防治策略提供依据。方法 2015年10月至2016年3月，选择浙江省从事牛羊饲养、运输、交易、屠宰、兽医、皮毛乳肉加工及海洋捕捞等布病重点职业人群，采集其全血5 ml，分离血清，采用RBPT和SAT进行实验室血清学检测，采用病例对照研究，利用多因素Logistic回归进行危险因素分析。结果 采集重点职业人员全血3 410份，其中105份血清SAT阳性，感染率为3.08%。男女性感染率分别为3.10%和3.02%，差异无统计学意义（χ²=0.010，P=0.970）。多因素Logistic回归分析发现，从不戴手套、手部有伤口或皲裂与布病发病有关。结论 从不戴手套、手部有伤口或皲裂是目前浙江省布病发病的高危因素；应开展健康教育和干预措施，提高从业人员的防护意识。

Objective To investigate the brucellosis infection and risk factors among occupational populations in Zhejiang province, and to provide scientific basis for preventive measures. Methods Occupational populations engaged in cultivation, transportation, trading, veterinarian services, fur making, marine fishing from October 2015 to March 2016 were selected for study. Rose bengal plate test (RBPT) and Serum agglutination test (SAT) were used for serological test. A case-control study was conducted to analyze the risk factors through logistic regression analysis. Results Among all 3 410 respondents, 105 were infected with brucellosis, with the infection rate of 3.08%. The difference of infection rate between male (3.10%) and female (3.02%) had no statistical significance (χ²=0.010, P=0.970). Multivariate logistic regression analysis showed that no wearing gloves and hand wound were found to be associated with the incidence of brucellosis. Conclusion Risk factors for the incidence of brucellosis in Zhejiang province were no wearing gloves and hand wound. Health education and intervention should be strengthened to improve the protection and awareness of brucellosis in occupational populations.