目的 调查黑龙江省牡丹江口岸地区鼠类巴尔通体感染情况。方法 2014年6月，在牡丹江口岸草地及绿化带利用夹夜法和鼠笼法共捕获鼠类33只，采集其脾样本；采用PCR方法检测鼠类巴尔通体感染情况，将阳性样本进行基因测序鉴定。结果 共检出巴尔通体核酸阳性样本6份，感染率为18.18%，其中5份分离自黑线姬鼠，1份分离自褐家鼠。牡丹江口岸至少存在4种基因型巴尔通体，包括Bartonella taylorii、B. phoceensis、对人类有致病性的B. grahamii及未定种巴尔通体，提示牡丹江口岸存在巴尔通体基因型多样性的特征。结论 黑龙江省牡丹江口岸鼠类存在巴尔通体感染，且携带人类致病性的巴尔通体，对人群有致病风险，应加强口岸地区的鼠类防控能力。
Objective To investigate Bartonella infection in rodents at Mudanjiang airport in Heilongjiang province, China. Methods A total of 33 rodents were captured by night trapping and cage method, and their spleen were aseptically collected to detect Bartonella by PCR. The positive samples obtained were further identified through gene sequencing. Results The results showed that 6 positive samples of Bartonella in all rodents,infection prevalence was 18.18%, 5 of which were isolated from Apodemus agrarius, one from Rattus norvegicus. Results showed that at least 4 subtypes of Bartonella were found at Mudanjiang airport, including B. taylorii, B. phoceensis, B. grahamii that are pathogenic to humans, as well as an undetermined species. The results suggested the genotype diversity of Bartonella at Mudanjiang airport. Conclusion The prevalence of Bartonella infection in rodents at Mudanjiang airport poses a risk of transmission to human, and appropriate control measures should be taken into consideration.
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