目的 研究Southern印记杂交方法检测鼠疫耶尔森菌（鼠疫菌）Pla片段的有效性。方法 采用Southern印记杂交技术与鼠疫菌核酸测定试剂盒两种方法对鼠疫菌Pla片段进行检测。结果 两种方法检测结果相同，Southern印记杂交技术可直观地观察pla基因拷贝数，杂交效率较高；实时荧光定量PCR检测结果为阳性。结论 Southern印记杂交技术适用于鼠疫菌Pla片段的检测。
Objective To determine the effectiveness of Southern blot method for detection of Pla fragment in Yersinia pestis. Methods Using Southern blot and Y. pestis quantitative real-time PCR kit to detect the Pla fragment. Results The results of Southern blot were same as real-time PCR kit. Southern blot allowed to see the number of pla gene copies directly, the efficiency of hybridization was high; PCR detection was also positive. Conclusion Southern blot is suitable for detection of Pla fragment of Y. pestis.
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