目的 通过实验室驯化的不同宿主型猫栉首蚤对宿主动物的吸血率和饱血率比较研究，为开展猫栉首蚤的传染病传播机制及媒介效能等研究提供依据。方法 观察刚刚羽化、30 min内羽化和1~4日龄的大白鼠宿主型、小白鼠宿主型和猫宿主型猫栉首蚤，在不同时间内对大白鼠、小白鼠和猫的吸血和饱血情况，并利用SAS 9.4软件对吸血率和饱血率进行统计学分析。结果 1 d内羽化及3、4日龄的大白鼠株与小白鼠株猫栉首蚤其吸血率差异无统计学意义；2~4日龄的猫栉首蚤饱血率差异有统计学意义（大白鼠株猫栉首蚤：χ2=136.206，P<0.001；小白鼠株猫栉首蚤：χ2=219.709，P<0.001）；大白鼠株及小白鼠株猫栉首蚤的吸血率和饱血率均随着吸血时间的延长呈递增趋势；猫株猫栉首蚤对3种供血动物的喜好顺序为猫 > 大白鼠=小白鼠，大白鼠株猫栉首蚤为大白鼠 > 猫 > 小白鼠，小白鼠株猫栉首蚤为小白鼠=猫 > 大白鼠。结论 羽化后不同时间、不同吸血时间及不同供血宿主均对猫栉首蚤的吸血率和饱血率产生影响，提示猫栉首蚤的适应能力强，可产生宿主分化并能发挥更大的媒介效能。
Objective To study the blood-sucking and blood-engorging rates of different host-types of cat fleas in laboratory, and provided experimental materials for further study on the transmission of infectious diseases and vectorial capacity of cat fleas. Methods The newly emerged, 30-min and 1 to 4 days old cat fleas which have adapted to lab rat, lab mouse and cat were allowed to feed on their corresponding hosts. The blood-sucking and blood-engorging rates were observed at different time points, and statistical analysis was performed with SAS 9.4 software. Results There was no significant difference in the rate of blood sucking between the rat-and mouse-adapted fleas with ages of 1, 3, and 4 days old. The difference of the rate of blood engorging between 2-4 days old fleas was significant, i. e. χ2=136.206, P<0.001 in the rat-adapted fleas, χ2=219.709, P<0.001 in the mouse-adapted fleas. The blood-sucking and blood-engorging rates of rat-and mouse-adapted fleas all increased with the time of blood-sucking. The order of host preference to three feeding animals was cat > rat=mouse in cat-adapted fleas, rat > cat > mouse in rat-adapted fleas, and mouse=cat > rat in mouse-adapted fleas. Conclusion Different blood-sucking time and different blood-supplying hosts all had an effect on the blood-sucking rate and blood-engorging rate of cat fleas, suggesting that the fleas were highly adaptable and can shift their hot preference and alter their vectorial capacity.
[1] 栗冬梅, 刘起勇, 俞东征, 等. 用PCR方法检出蚤类携带巴尔通体[J]. 中国媒介生物学及控制杂志, 2005, 16(1):5-8.
[2] Higgins JA,Radulovic S,Schriefer ME,et al. Rickettsia felis:a new species of pathogenic Rickettsia isolated from cat fleas[J]. J Clin Microbiol, 1996, 34(3):671-674.
[3] Rolain JM,Franc M,Davoust B,et al. Molecular detection of Bartonella quintana,B. koehlerae,B. henselae,B. clarridgeiae, Rickettsia felis,and Wolbachia pipientis in cat fleas, France[J]. Emerg Infect Dis, 2003, 9(3):338-342.
[4] Kelly PJ, Meads NA, Theobald A, et al. Rickettsia felis, Bartonella henselae, and B. clarridgeiae,New Zealand[J]. Emerg Infect Dis, 2004, 10(5):967-968.
[5] Finkelstein JL,Brown TP,O'Reilly KL,et al. Studies on the growth of Bartonella henselae in the cat flea (Siphonaptera:Pulicidae)[J]. J Med Entomol, 2002, 39(6):915-919.
[6] Woods JE, Brewer MM, Hawley JR, et al. Evaluation of experimental transmission of Candidatus Mycoplasma haemominutum and Mycoplasma haemofelis by Ctenocephalides felis to cats[J]. Am J Vet Res, 2005, 66(6):1008-1012.
[7] Tsai KH,Lu HY,Huang JH,et al. Rickettsia felis in cat fleas in Taiwan[J]. Vector Borne Zoonotic Dis, 2009, 9(5):561-563.
[8] 范明远,栾明春. 新发现的蚤传斑点热立克次体病[J]. 中国人兽共患病学报, 2008, 24(1):74-77.
[9] McTier TL,Shanks DJ,Jernigan AD,et al. Evaluation of the effects of selamectin against adult and immature stages of fleas (Ctenocephalides felis felis)on dogs and cats[J]. Vet Parasitol, 2000, 91(3/4):201-212.
[10] Schenker R,Tinembart O,Humbert-Droz E,et al. Comparative speed of kill between nitenpyram,fipronil,imidacloprid, selamectin and cythioate against adult Ctenocephalides felis (Bouché)on cats and dogs[J]. Vet Parasitol, 2003, 112(3):249-254.
[11] 刘泉,陈建新. 蚤类吸血行为和血液消化的研究概述[J]. 中国媒介生物学及控制杂志, 1994, 5(4):309-319.
[12] 吴丹丹. 猫栉首蚤生物学及不同地理种群系统进化研究[D]. 哈尔滨:东北林业大学, 2010.
[13] 孟凤霞,刘起勇,冯延龙,等. 印鼠客蚤实验室种群的羽化规律研究[J]. 寄生虫与医学昆虫学报, 2008, 15(2):86-88.
[14] 张洪杰,吴厚永,刘泉. 方形黄鼠蚤松江亚种在不同动物体上吸血率的观察[J]. 地方病通报, 1991, 6(4):107-108.
[15] Cadiergues MC, Hourcq P,Cantaloube B,et al. First bloodmeal of Ctenocephalides felis felis(Siphonaptera:Pulicidae)on cats:time to initiation and duration of feeding[J]. J Med Entomol, 2000, 37(4):634-636.
[16] 林杰,孟凤霞,吴丹丹. Y型嗅觉仪在蚤类对宿主选择性研究中的应用[J]. 中国媒介生物学及控制杂志, 2011, 22(2):103-106, 107.
[17] Wang YG,Tian J,Liu QY,et al. Influence of bloodmeal host on blood feeding,egg production,and offspring sex ratio of Ctenocephalides felis felis(Siphonaptera:Pulicidae)[J]. J Med Entomol, 2016, 53(4):888-893.
[18] 杨小冉,刘起勇,崔步云,等. BALB/c及昆明小鼠的汉赛巴尔通体实验室感染模型[J]. 中国媒介生物学及控制杂志, 2008, 19(1):41-43.
[19] 刘小闪,刘起勇. 汉赛巴尔通体媒介和宿主的实验室研究进展[J]. 中国媒介生物学及控制杂志, 2006, 17(2):157-159.
[20] 孟凤霞,刘起勇. 杀蚤剂的使用历史及蚤的综合防制[J]. 中国媒介生物学及控制杂志, 2004, 15(5):341-343.
