湖北省随州市2016年3例布鲁氏菌病流行病学调查及菌株鉴定

赵忠智, 聂丹文, 韩秀瑞, 刘志国, 朴东日, 姜海, 田国忠, 赵鸿雁, 崔步云

doi:10.11853/j.issn.1003.8280.2017.02.017

中国媒介生物学及控制杂志 >

2017 , Vol. 28 >Issue 2: 163 - 165

DOI: https://doi.org/10.11853/j.issn.1003.8280.2017.02.017

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湖北省随州市2016年3例布鲁氏菌病流行病学调查及菌株鉴定

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1 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室, 感染性疾病诊治协同创新中心, 北京 102206;
2 青海省地方病预防控制所, 西宁 811602;
3 随州市疾病预防控制中心, 湖北随州 441300

赵忠智,男,硕士,医师,主要从事鼠疫布病的分子病原学检验相关研究,Email:zhzhzh1026@sohu.com

收稿日期: 2016-12-09

网络出版日期: 2017-04-20

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Epidemiological investigation and pathogen identification in three cases of brucellosis from Suizhou city, Hubei province in 2016

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1 State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Beijing 102206, China;
2 Qinghai Institute for Endemic Diseases Prevention and Control;
3 Suizhou Center for Disease Control and Prevention

Received date: 2016-12-09

Online published: 2017-04-20

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摘要

目的对湖北省随州市就诊的3例疑似布鲁氏菌病（布病）患者进行个案调查，并对分离的3株疑似布鲁氏菌鉴定分型。方法应用布病流行病学调查表进行现场调查，采用布鲁氏菌常规鉴定分型方法和噬菌体裂解试验进行检测，并比较BCSP31-PCR法（以布鲁氏菌属特异基因BCSP31为靶基因的检测方法）、AMOS-PCR（根据电泳条带鉴别布鲁氏菌牛种1、2、4型、羊种布鲁氏菌、猪种l型、绵羊附睾种）及实时荧光定量PCR 3种分子分型种型鉴定方法。结果 3例患者均由密切接触病畜及其肉制品感染，常规检测方法鉴定为羊种布鲁氏菌3型，多种PCR方法检测获得同样结果。结论经常规和PCR鉴定分型研究确定，随州市布病病例分离株为羊种布鲁氏菌3型。

关键词： 布鲁氏菌; 个案调查; 聚合酶链式反应; 鉴定

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赵忠智, 聂丹文, 韩秀瑞, 刘志国, 朴东日, 姜海, 田国忠, 赵鸿雁, 崔步云 . 湖北省随州市2016年3例布鲁氏菌病流行病学调查及菌株鉴定[J]. 中国媒介生物学及控制杂志, 2017 , 28(2) : 163 -165 . DOI: 10.11853/j.issn.1003.8280.2017.02.017

Abstract

Objective Three suspected brucellosis cases were investigated in Suizhou city of Hubei province, and three suspected Brucella strains were identified. Methods Field investigation was applied to finish Brucella epidemiological survey, used Brucella conventional appraisal classification method and the phage cracking test for testing. BCSP31-PCR method (Based on Brucella genus specific gene BCSP31 target gene detection method), AMOS-PCR (based on electrophoresis banding identify B. abortus biovar 1, 2, 4 of Brucella, B. melitensis, B. ovis and B. suis biovar 1) and Real-time PCR, three molecular classification method were compared. Results Three patients were infected by close contact with sick animals and their meat, conventional identification and a variety of polymerase chain reaction (PCR) had the same results, B. melitensis biovar 3. Conclusion Brucella melitensis biovar 3 was identified as the pathogen of the three cases from Suizhou city and cases were mainly caused by direct contact with sick animals and their meat.

Key words： Brucella; Individual survey; Polymerase chain reaction; Identification

参考文献

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