ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
收稿日期: 2016-10-24
网络出版日期: 2017-04-20
基金资助
北京市优秀人才培养资助项目（2014000052580G290）；北京市朝阳区疾病预防控制中心自选项目（Cycdc-2015-271103）
First report of Liaoning virus from mosquitoes in Chaoyang district, Beijing
Received date: 2016-10-24
Online published: 2017-04-20
Supported by
Supported by the Beijing Outstanding Young Talent Training Project(No. 2014000052580G290) and the Research Foundation of Chaoyang District Center for Disease Control and Prevention (No. Cycdc-2015-271103)
目的 了解北京市朝阳区蚊虫携带辽宁病毒（LNV）情况。方法 按照《病媒生物密度监测方法 蚊虫》（GB/T 23797－2009）中的方法，于2014－2015年采用CO2诱蚊灯采集蚊虫，利用反转录聚合酶链式反应检测LNV核酸，采用Mega 6.0软件进行进化分析。结果 2014－2015年共采集蚊虫9 811只，其中淡色库蚊为优势种，占捕获总数的91.41%（8 968/9 811）。标本编号BJCY14007的淡色库蚊核酸经检测为LNV阳性。基于LNV第10节段的分子特征显示，LNV分支为两个进化群血清Ⅰ型和血清Ⅱ型，BJCY14007与NE97-12较为接近，属血清Ⅰ型。结论 北京市首次报道从蚊虫中检出LNV。因LNV有潜在的致病性，应注意其分布及变化情况，且有必要在人群及宿主动物中开展血清学调查。
唐承军, 葛军旗, 张洪江, 付士红, 李元元, 徐潮, 王会平, 张政 . 北京市朝阳区首次从蚊虫标本中检出辽宁病毒[J]. 中国媒介生物学及控制杂志, 2017 , 28(2) : 113 -116 . DOI: 10.11853/j.issn.1003.8280.2017.02.004
Objective To investigate Liaoning virus(LNV) in mosquitoes in Chaoyang district, Beijing. Methods According to the Surveillance for Vector Density-Mosquito (GB/T 23797-2009), mosquitoes were collected by CO2 traps in 2014 and 2015. Reverse transcription-polymerase chain reaction was used to detect LNV. Molecular characteristics were carried out by Mega 6.0. Results A total of 9 811 mosquitoes were collected and Culex pipiens pallens (8 968/9 811, 91.41%) was the main species. The sample BJCY14007 was positive for LNV. LNV has two branches (serotypeⅠand serotypeⅡ) based on the molecular characteristics of the segment 10. BJCY14007 was more related to NE97-12 (representative strain, serotypeⅠ). Conclusion The LNV was firstly detected in Beijing. It deserves further investigation LNV prevalence in mosquito, human population and other hosts for risk assessment.
Key words： Arbovirus; Liaoning virus; Molecular characteristics; Beijing
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