应用细胞色素C氧化酶亚基Ⅰ和16S rRNA基因鉴定浙江口岸鼠类及其序列特征分析

齐润姿, 王董, 普丽娅, 赵欣, 高雪萌, 马翔, 余斌, 张晓龙

doi:10.11853/j.issn.1003.8280.2017.02.002

中国媒介生物学及控制杂志 >

2017 , Vol. 28 >Issue 2: 104 - 107

DOI: https://doi.org/10.11853/j.issn.1003.8280.2017.02.002

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应用细胞色素C氧化酶亚基Ⅰ和16S rRNA基因鉴定浙江口岸鼠类及其序列特征分析

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1 浙江出入境检验检疫局, 杭州 310016;
2 中国检验检疫科学研究院, 北京 100176;
3 温州出入境检验检疫局, 浙江温州 325027

齐润姿,女,博士,主治医师,副处长,主要从事口岸传染病防控和病媒生物防制工作,Email:724859571@qq.com

收稿日期: 2016-10-24

网络出版日期: 2017-04-20

基金资助

国家质检总局科技计划项目（2015IK303）

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Identification of rodent species in Zhejiang port using cytochrome C oxidase subunitⅠ and 16S rRNA gene and analysis of sequence characteristics

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1 Zhejiang Entry-Exit Inspection and Quarantine Bureau, Hangzhou 310016, Zhejiang Province, China;
2 Chinese Academy of Inspection and Quarantine;
3 Wenzhou Entry-Exit Inspection and Quarantine Bureau

Received date: 2016-10-24

Online published: 2017-04-20

Supported by

Supported by the General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China Scientific Research Subject(No. 2015IK303)

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摘要

目的鉴定浙江口岸鼠类，并对其序列特征进行分析。方法于2015年4－10月在浙江口岸捕获鼠类，取其肝脏，对捕获鼠类的线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）、16S rRNA序列片段进行扩增，鉴定鼠种并构建系统发育树。结果浙江口岸捕获的鼠类为褐家鼠和小家鼠。COⅠ基因变异率大于16S rRNA基因，均以转换为主，且COⅠ基因以同义突变为主。结论 COⅠ和16S rRNA基因均可作为鼠类鉴定的分子标记。

关键词： 鼠类; 系统发育树; 鉴定

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齐润姿, 王董, 普丽娅, 赵欣, 高雪萌, 马翔, 余斌, 张晓龙 . 应用细胞色素C氧化酶亚基Ⅰ和16S rRNA基因鉴定浙江口岸鼠类及其序列特征分析[J]. 中国媒介生物学及控制杂志, 2017 , 28(2) : 104 -107 . DOI: 10.11853/j.issn.1003.8280.2017.02.002

Abstract

Objective To identify rodent species in Zhejiang port and analyze the sequence characteristics. Methods The livers were obtained from rodents captured during April to October in 2015. The cytochrome C oxidase subunitⅠ(COⅠ) and 16S rRNA gene sequences of rodents were amplified. The species were confirmed and the phylogenetic trees were constructed. Results There were two species, Rattus norvegicus and Mus musculus in Zhejiang port. The mutation rate of COⅠgene was greater than that of 16S rRNA gene. Most of the mutation was transition and synonymous mutation. Conclusion Both COⅠ gene and 16S rRNA gene, as molecular markers, could be applied to identify rodent species.

Key words： Rodents; Phylogenetic trees; Identification

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