ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
收稿日期: 2016-10-31
网络出版日期: 2017-04-20
基金资助
国家科技重大专项（2012ZX10004-219）
Identification and distribution of Apodemus species with DNA barcoding in China
Received date: 2016-10-31
Online published: 2017-04-20
Supported by
Supported by the National Science and Technology Major Project of China (No. 2012ZX10004-219)
目的 利用DNA条形码技术对采自我国22个省的姬鼠属样本进行鉴定，调查其分类及在我国的分布情况，为我国鼠传疾病的防控与监测提供数据支持。方法 对鼠类样本的线粒体细胞色素C氧化酶亚基Ⅰ（COⅠ）基因片段进行扩增并测序，构建系统发育树，并与GenBank中相关序列进行比对。结果 119份姬鼠属样本经PCR检测均获得有效COⅠ基因扩增片段，通过构建分子进化树得到8个分支，与GenBank中的数据进行比对，可确定为8个独立的鼠种，分别为黑线姬鼠、齐氏姬鼠、朝鲜姬鼠、大耳姬鼠、澜沧江姬鼠、中华姬鼠、乌拉尔姬鼠及1个姬鼠属未定种。结论 通过对鼠类样本的准确鉴定，可确定这些种在我国较为准确的分布情况，修订了原基于形态鉴定的物种分布。
刘蓉蓉, 葛德燕, 鲁亮, 夏琳, 刘起勇, 杨奇森 . 中国姬鼠属种类的DNA条形码鉴定及其分布[J]. 中国媒介生物学及控制杂志, 2017 , 28(2) : 97 -103 . DOI: 10.11853/j.issn.1003.8280.2017.02.001
Objective To investigate the taxonomy and distribution of Apodemus species in China, and to provide supportive data for prevention and control of rodent borne disease. Methods DNA barcoding was employed to identify Apodemus samples collected from 22 provincial areas in China. Genomic DNA was extracted from tissue samples for PCR amplification of the mitochondrial cytochrome C oxidase subunitⅠ (COⅠ) gene, followed by sequencing. The obtained sequences were compared with the related sequences in GenBank. Then a phylogenetic tree was constructed. Results COⅠ gene fragment were amplified from 119 Apodemus samples, and they were sorted into eight branches through phylogenetic reconstruction. Compared with the data in the GenBank, the collected samples were identified as eight independent species: Ap. agrarius, Ap. chevrieri, Ap. peninsulae, Ap. latronum, Ap. ilex, Ap. draco, Ap. uralensis and an undefined species. Conclusion The accurate distribution of Apodemus species in China can be determined by effective identification of samples through DNA barcoding. In addition, the results revised the Apodemus species distribution based on the morphological identification.
Key words： DNA barcoding; Apodemus; Species identification; Distribution
[1] 余向华,张孝和,倪庆翔,等. 鼠传疾病流行病学研究进展[J]. 中国媒介生物学及控制杂志,2015,26(6):634-636,644.
[2] 王芹,李建东,张全福,等. 2014年全国肾综合征出血热监测总结和疫情分析[J]. 疾病监测,2016,31(3):192-199.
[3] 杨海,蔡正华,宗红. 试论姬鼠起源和姬鼠型出血热[J]. 国际病毒学杂志,2009,16(3):94-97.
[4] 黄文几. 中国啮齿类[M]. 上海:复旦大学出版社,1995:122-130.
[5] 张荣祖. 中国哺乳动物分布[M]. 北京:中国林业出版社,1997: 188-193.
[6] Wilson DE,Reeder DM. Mammal species of the world:a taxonomic and geographic reference[M]. 3rd ed. Baltimore:The Johns Hopkins University Press,2005:1259-1280.
[7] 郑智民,姜志宽,陈安国. 啮齿动物学. 第2卷[M]. 2版. 上海: 上海交通大学出版社,2012:57-74.
[8] 王应祥. 中国哺乳动物种和亚种分类名录与分布大全[M]. 北京:中国林业出版社,2003:193-196.
[9] 蒋志刚,马勇,吴毅,等. 中国哺乳动物多样性[J]. 生物多样性,2015,23(3):351-364.
[10] Liu XM,Wei FW,Li M,et al. Molecular phylogeny and taxonomy of wood mice(genus Apodemus Kaup,1829)based on complete mtDNA cytochrome b sequences,with emphasis on Chinese species[J]. Mol Phylogenet Evol,2004,33(1):1-15.
[11] 蒋学龙,王应祥. 长尾姬鼠分类地位的探讨[J]. 动物学研究, 2000,21(6):473-478.
[12] 杨海,王戬. 姬鼠系统分类研究[J]. 中华地方病学杂志, 2008,27(5):587-589.
[13] 何锴,王文智,李权,等. DNA条形码技术在小型兽类鉴定中的探索:以甘肃莲花山为例[J]. 生物多样性,2013,21(2): 197-205.
[14] Li J,Zheng X,Cai YS,et al. DNA barcoding of Murinae (Rodentia:Muridae) and arvicolinae(Rodentia:Cricetidae) distributed in China[J]. Mol Ecol Res,2014,15(1):153-167.
[15] Ondrejicka DA,Locke SA,Morey K,et al. Status and prospects of DNA barcoding in medically important parasites and vectors[J]. Trends Parasitol,2014,30(12):582-591.
[16] 金圣浩,鲁亮,闫东,等. DNA条形码技术在河北省坝上地区夜行鼠鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2015,26(1):55-57.
[17] Tamura K,Stecher G,Peterson D,et al. Mega 6:molecular evolutionary genetics analysis version 6.0[J]. Mol Biol Evol, 2013,30(12):2725-2729.
[18] Atteson K. The performance of Neighbor-Joining methods of phylogenetic reconstruction[J]. Algorithmica,1999,25(2/3): 251-278.
[19] Gascuel O,Steel M. Neighbor-Joining revealed[J]. Mol Biol Evol,2006,23(11):1997-2000.
[20] Altschul SF,Madden TL,Schäffer AA,et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Res,1997,25(17):3389-3402.
[21] Lobo I. Basic local alignment search tool(BLAST)[J]. J Mol Biol, 1990,215(3):403-410.
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