调查研究

登封市2013年小肠结肠炎耶尔森菌生物学特性研究

展开
  • 1. 登封市卫生防疫站, 河南 登封 452470;
    2. 河南省疾病预防控制中心, 郑州 450016
张胜勇,男,主管检验师,主要从事微生物学检验,Email:zsy0371@163.com

收稿日期: 2016-06-26

  网络出版日期: 2016-12-20

基金资助

国家科技重大专项(2013ZX10004203-002-002)

Investigation on biological characteristics of Yersinia enterocolitica in Dengfeng city, 2013

Expand
  • 1. Dengfeng Hygiene and Anti-epidemic Station, Dengfeng 452470, Henan Province, China;
    2. Henan Center for Disease Control and Prevention

Received date: 2016-06-26

  Online published: 2016-12-20

Supported by

Supported by the National Science and Technology Major Project of China(No. 2013ZX10004203-002-002)

摘要

目的 了解登封市2013年小肠结肠炎耶尔森菌生物学特性及宿主分布特征。方法 用传统培养方法分离小肠结肠炎耶尔森菌,并进行生化鉴定、血清分型和毒力基因PCR检测。结果 2013年采集标本共1 138份,分离出小肠结肠炎耶尔森菌169株,总检出率为14.85%;其中猪标本检出率高达36.55%(114/394);生物型3型占86.39%(146/169),均为O:3血清型;毒力基因ail+ystA+yadA+virF+占81.06%(137/169),ail+ystA+占5.33%(9/169),ystB+占12.43%(21/169),ail-ystA-ystB-yadA-virF-占1.18%(2/169)。结论 猪是小肠结肠炎耶尔森菌的优势宿主;小肠结肠炎耶尔森菌的血清型以O:3型为主。

本文引用格式

张胜勇, 李洪民, 王德祥, 张锦, 夏胜利 . 登封市2013年小肠结肠炎耶尔森菌生物学特性研究[J]. 中国媒介生物学及控制杂志, 2016 , 27(6) : 585 -586 . DOI: 10.11853/j.issn.1003.8280.2016.06.016

Abstract

Objective To investigate the biological characteristics and host distribution of Yersinia enterocolitica in Dengfeng, 2013. Methods Yersinia enterocolitica were isolated by tradition culture method. These bacteria were analyzed by the systematic biochemistry, serotype, and PCR detection methods. Results There were 1 138 samples collected in 2013. The isolation rate of Y. enterocolitica was 14.85%(169/1 138), the isolation rate of swine was 36.55%(144/394). In total, 146(86.39%), were classified into biology type 3 and O:3. The toxicity gene distribution characters were as follow:81.06%(137/169) of them were ail+, ystA+, yadA+, virF+, 5.33%(9/169) were ail+, ystA+, 12.43%(21/169) were ystB+, 1.18%(2/169) were ail-, ystA-, ystB-, yadA-, virF-. Conclusion Swine were important host as pathogenic Y. enterocolitica. O:3 was the major serotype.

参考文献

[1] 布坎南RE,吉本斯NE. 伯杰细菌鉴定手册[M]. 中国科学院微生物研究所,译. 8版. 北京:科学出版社, 1984:462.
[2] Visser LG,Annema A,Van Furth R. Role of Yops in inhibition of phagocytosis and killing of opsonized Yersinia enterocolitica by human granulocytes[J]. Infect Immun, 1995, 63(7):2570-2575.
[3] 汪华,景怀琦,朱凤才,等. 小肠结肠炎耶尔森氏菌[M]. 北京:人民卫生出版社, 2004:16-18.
[4] 孙殿斌,靳荣华,庞炜英,等. 我国首次发生小肠结肠炎耶氏菌病暴发流行[J]. 中国人兽共患病杂志, 1987, 3(5):2-4.
[5] 李洪民,赵嘉咏,张胜勇,等. 2009-2012年登封市小肠结肠炎耶尔森菌分布调查[J]. 中国人兽共患病学报, 2015, 31(3):281-283.
[6] 张胜勇,李洪民,梅志敏,等. 2011年登封市小肠结肠炎耶尔森菌生物学特性研究[J]. 中国地方病防治杂志, 2015, 30(3):176-177.
[7] 张胜勇,梅志敏,李洪民,等. 登封市一株小肠结肠炎耶尔森菌带毒株分析[J]. 中国媒介生物学及控制杂志, 2015, 26(4):409-410.
[8] 穆玉姣,赵嘉咏,郭晓醇,等. 从生猪中分离小肠结肠炎耶尔森菌病原学研究[J]. 中国人兽共患病学报, 2012, 28(12):1220-1222, 1232.
[9] Liang JR, Wang X, Xiao YC. Prevalence of Yersinia enterocolitica in pigs slaughtered in Chinese abattoirs[J]. Appl Environ Microbiol, 2012, 78(8):2949-2956.
[10] 孟真,石国祥,程苏云,等. 浙江省部分地区小肠结肠炎耶尔森菌(动物株)分子生物学特征初探[J]. 中国卫生检验杂志, 2007, 17(1):60-62, 105.
[11] 李孟磊,穆玉娇,党李成,等. 河南省2007-2010年小肠结肠炎耶尔森菌监测分析[J]. 中国人兽共患病学报, 2013, 29(3):312-315.
[12] 曹严华,夏胜利,顾玲,等. 2004-2005年我国部分地区小肠结肠炎耶尔森菌宿主分布调查[J]. 中国媒介生物学及控制杂志, 2007, 18(3):238-240.
[13] 肖玉春,梁俊容,古文鹏,等. 不同动物宿主小肠结肠炎耶尔森氏菌分离及结果分析[J]. 中国人兽共患病学报, 2012, 28(5):418-420.

文章导航

/