收稿日期: 2016-07-26
网络出版日期: 2016-12-20
基金资助
湖南省自然科学基金(12JJ2017);湖南省质量技术监督局科研计划(2016KYJH29)
Insecticidal and repellent activity of ethanol extracts from 4 plants against Musca domestica (Linnaeus) (Diptera: Muscidae)
Received date: 2016-07-26
Online published: 2016-12-20
Supported by
Supported by the Natural Science Foundation of Hunan Province of China(No. 12JJ2017) and the Scientific Research Project of Hunan Quality and Technique Supervision Bureau(No. 2016KYJH29)
目的 探究香樟叶、天竺桂叶、侧柏叶和雪松叶的乙醇提取物对家蝇的生物活性,为植物源灭蝇剂的开发提供理论依据。方法 采用白糖载药饲虫法测定4种植物乙醇提取物对家蝇成虫的毒杀作用,采用滤纸药膜法测试4种植物乙醇提取物对家蝇3龄幼虫的驱避作用。结果 香樟叶、天竺桂叶、侧柏叶和雪松叶的乙醇提取物对家蝇的24 h半数致死浓度(LC50)分别为7.58、16.02、44.08和38.24 mg/ml。在剂量为1.572 mg/cm2时,4种植物乙醇提取物对家蝇3龄幼虫的驱避率:香樟叶为93.33%,天竺桂叶为88.33%,侧柏叶为58.33%,雪松叶为60.00%。结论 4种植物提取物对家蝇有较好的毒杀和驱避活性,可用于研发绿色环保灭蝇剂。
何建国, 熊国红, 徐颖 . 4种植物乙醇提取物对家蝇的毒杀及驱避活性[J]. 中国媒介生物学及控制杂志, 2016 , 27(6) : 542 -545 . DOI: 10.11853/j.issn.1003.8280.2016.06.004
Objective We studied the insecticidal and repellent activity of ethanol extracts from 4 plants against Musca domestica so as to provide theoretical basis for eco-friendly Muscacide. Methods The larvicidal activity of 4 plant ethanol extracts against M. domestica was determined by drug loaded sugar feeding method, and the repellent activity of ethanol extracts from 4 plants against M. domestica was evaluated using the impregnated paper assays. Results The results showed that the LC50 values of ethanol extracts from the leaves of Cinnamomum caphoran, C. pedunculatum, Platycladus orientalis, and Cedrus deodara against M. domestica were 7.58, 16.02, 44.08, 38.24 mg/ml, respectively. At the concentration of 1.572 mg/cm2, the repellent percentages of ethanol extracts from the leaves of C. caphoran, C. pedunculatum, P. orientalis, and C. deodara were 93.33%, 88.33%, 58.33%, and 60.00%, respectively. Conclusion The results suggested that the 4 plant ethanol extracts possess high insecticidal and repellent activity against M. domestica, they have a great potential to be developed into a eco-friendly Muscacide.
Key words: Plant extracts; Musca domestica; Insecticidal activity; Repellent activity
[1] 贾海燕, 刘小可, 苗元, 等. 食堂越冬家蝇体表携带细菌的初步研究[J]. 北京农学院学报, 2011, 26(1):13-15, 41.
[2] 吕锡宏, 费胜军, 许海根, 等. 上海市松江区家蝇对5种杀虫剂的抗性测定及分析[J]. 医学动物防制, 2015, 31(4):365-367.
[3] 魏绪强, 李宗麟, 潘京海, 等. 北京市东城区家蝇对溴氰菊酯抗性风险评估与预报[J]. 中国媒介生物学及控制杂志, 2013, 24(5):402-405.
[4] 董存柱, 吴清照, 徐汉虹, 等. 海南40种植物甲醇提取物对家蝇的活性筛选[J]. 江西农业大学学报, 2011, 33(3):476-481.
[5] 周利娟, 黄继光, 徐汉虹, 等. 从华蟹甲草中分离的两种活性成分对家蝇的杀虫活性[J]. 昆虫学报, 2006, 49(1):74-79.
[6] 聂霄艳, 邓永学, 王进军, 等. 花椒精油和麻素对赤拟谷盗成虫的控制作用[J]. 中国粮油学报, 2008, 23(4):185-188.
[7] Rossi YE,Canavoso L, Palacios SM. Molecular response of Musca domestica L. to Mintostachys verticillata essential oil,(4R) (+)-pulegone and menthone[J]. Fitoterapia, 2012, 83(2):336-342.
[8] Rossi YE,Palacios SM. Insecticidal toxicity of Eucalyptus cinerea essential oil and 1,8-cineole against Musca domestica and possible uses according to the metabolic response of flies[J]. Ind Crops Prod, 2015, 63:133-137.
[9] Sharma PP, Pardeshi AB, Vijigiri D. Bioactivity of some medicinal plant extracts against Musca domestica L.[J]. J Ecobiotechnol, 2011, 3(9):14-16.
[10] Morey RA, Khandagle AJ. Bioefficacy of essential oils of medicinal plants against housefly, Musca domestica L.[J]. Parasitol Res, 2012, 111(4):1799-1805.
[11] Kumar P,Mishra S,Malik A,et al. Compositional analysis and insecticidal activity of Eucalyptus globulus(family:Myrtaceae) essential oil against housefly(Musca domestica)[J]. Acta Trop, 2012, 122(2):212-218.
[12] 徐河山. 植物源杀虫剂作用方式和机理的研究进展[J]. 热带医学杂志, 2006, 6(6):743-744, 716.
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