目的 调查北京市顺义区2010－2014年人间布鲁氏菌病（布病）的感染情况，了解流行病学特征，为制定防控策略提供科学依据。方法 对布病重点职业人群的血清标本进行虎红平板凝集试验检测，阳性或可疑标本进一步利用试管凝集试验判定。对高危职业人群布病感染情况及人间发病情况进行描述性分析。结果 2010－2014年顺义区共报告病例35例，呈逐年上升趋势（χ²=15.002，P < 0.05）；农民发病病例占51.43%，71.43%的患者发病前有牛、羊接触史。累计检测高危人群1 025人，阳性20人，阳性率为1.95%，重点职业人群布病抗体阳性率呈逐年下降趋势（χ²=16.960，P < 0.05），其中直接接触牛、羊的职业人群布病抗体阳性率分别为4.37%和0.54%，差异有统计学意义（χ²=6.031，P < 0.05）；农村地区、城区及城乡结合部职业人群阳性率分别为3.21%和0.48%，两地区布病抗体阳性率差异有统计学意义（χ²=8.807，P < 0.05）。结论 顺义区人间布病发病呈上升趋势，直接接触牛的人群为高危职业人群，应加强对顺义区重点职业人群布病的监测和健康宣传教育。

Objective To understand the infection and epidemiological characteristics of human Brucellosis in Shunyi district of Beijing, in 2010-2014, and provide scientific evidence for the prevention and control of the disease. Methods Rose-Bengal plate agglutination test were used for preliminary screening to 1 025 blood samples of Brucellosis high risk groups in Shunyi district from 2010 to 2014, and standard tube agglutination test were used for those preliminary screening positive blood samples to make a definite diagnosis for Brucellosis. Results A total of 35 cases were reported in 2010-2014, and there was an increasing trend during this period (χ²=15.002, P < 0.05); the percent of patients with Brucellosis for farmers was 51.43%, and there were 71.43% of cases that contacted with sheep or cattle before Brucellosis onset. Serological surveillance among 1 025 high dangerous populations was conducted in Shunyi, and there were 20 positive people of Brucellosis, that the positive rates was 1.95%, and there was a descending trend in 2010-2014 (χ²= 16.960, P < 0.05). The positive rates of direct contact with cattle and sheep were 4.37% and 0.54%, respectively, and there was a significant difference between those two groups (χ²=6.031, P < 0.05). The positive rates of rural area and rural-urban were 3.21% and 0.48% respectively, and there was a significant difference between those two groups (χ²=8.807, P < 0.05). Conclusion There was an increasing tendency for Brucellosis in Shunyi district, and the people with direct contact with cattle were the high risk population of Brucellosis, we should enhance surveillance and health education of prevention and control for the high risk populations of Brucellosis in Shunyi district.