收稿日期: 2016-04-11
网络出版日期: 2016-08-20
Analysis of cytochrome C oxidase subunitⅠgene of unknown and damaged fly species captured at Henan port
Received date: 2016-04-11
Online published: 2016-08-20
张勤, 王晓梦, 岳巧云, 邱德义, 高洁, 张芳, 王凯 . 河南口岸截获未知蝇种细胞色素C氧化酶亚基Ⅰ基因分析[J]. 中国媒介生物学及控制杂志, 2016 , 27(4) : 354 -357 . DOI: 10.11853/j.issn.1003.8280.2016.04.010
Objective To identify the unknown fly species with severely damaged morphological characteristics intercepted from Henan port. Methods DNA barcoding based on cytochrome C oxidase subunitⅠ gene was used to amplify and analyze the DNA of the unknown fly species. Results DNA barcoding and sequences analysis showed that the unknown fly species were identified as Chrysomya rufifacies. Conclusion DNA barcoding can partly compensate for the limitations of traditional morphological identification and has broad application prospects in the identification of medical vectors.
Key words: DNA barcoding; Molecular identification; Chrysomya rufifacies
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