ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
收稿日期: 2016-03-01
网络出版日期: 2016-08-20
An investigation on the harborage of three pathogens in small mammals in Beijing area, 2013
Received date: 2016-03-01
Online published: 2016-08-20
目的 了解恙虫病、莱姆病、巴贝西虫病3类疾病病原体在北京市各区小兽中的感染及分布情况。方法 2013年4－10月，采用夹夜法捕获小兽并解剖获取其组织样本，采用普通PCR与实时荧光定量PCR方法，检测小兽组织中恙虫病东方体、伯氏疏螺旋体以及巴贝西虫的特异性目的基因。结果 2013年北京地区500份小兽样本中恙虫病东方体阳性率为2.40%，来自6个区，以海淀区最高（21.43%），其他区恙虫病东方体感染率为2.04%~5.00%；巴贝西虫阳性率为6.40%，来自11个区，以海淀区最高（21.43%），其他区巴贝西虫感染率为1.96%~16.67%；检出恙虫病东方体与巴贝西虫共同感染样本4份，复合感染率为0.80%；所有样本中均未检测到伯氏疏螺旋体。结论 北京地区小兽中恙虫病东方体的感染不仅局限于发现病例的地区，应进一步加强全市人间病例以及宿主和媒介的监测工作，巴贝西虫小兽间感染在分布范围和感染率方面均高于恙虫病东方体，应加强对基层医生的培训并及时开展高危人群的监测与健康教育。
吕燕宁, 李良辰, 窦相峰, 陈丽娟, 张秀春, 关增智, 孙玉兰, 黎新宇, 王全意 . 2013年北京地区小兽中3种病原携带状况调查[J]. 中国媒介生物学及控制杂志, 2016 , 27(4) : 336 -340 . DOI: 10.11853/j.issn.1003.8280.2016.04.006
Objective To investigate the pathogen harborage in small mammals in Beijing area. Methods Night trapping method was used to capture the small mammals in Beijing area and the organ tissue was obtained from the trapped small mammals. PCR and Real-time PCR methods were used to detect the specific gene of Orientia tsutsugamushi, Borrelia burgdorferi and Babesia in the small mammals tissue. Results In 500 samples obtained from Beijing area in 2013, the positive rate of O. tsutsugamushi was 2.40% which was distributed in 6 districts, and the highest was 21.43% in Haidian district. The positive rate of Babesia was 6.40% which was distributed in 11 districts, and the highest was 21.43% in Haidian district. Four samples were coinfected with O. tsutsugamushi and Babesia, and the coninfection rate was 0.80%. The B. burgdorferi was not detected in any of the rodents. Conclusion The O. tsutsugamushi harborage in small mammals in Beijing was not restricted in the area where the human cases were reported, and the surveillance of human cases, animal hosts and vectors should be reinforced in Beijing. The Babesia harborage was higher than O. tsutsugamushi both in distribution and infection rate, therefore the training of the fundamental medical practitioners and the surveillance and health education of the high risk group should be carried out in time.
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