粗野粉蝇的DNA条形码和形态鉴定

魏晓雅, 邱德义, 岳巧云, 刘德星

doi:10.11853/j.issn.1003.8280.2016.04.003

中国媒介生物学及控制杂志 >

2016 , Vol. 27 >Issue 4: 326 - 329

DOI: https://doi.org/10.11853/j.issn.1003.8280.2016.04.003

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粗野粉蝇的DNA条形码和形态鉴定

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中山出入境检验检疫局检验检疫技术中心, 广东中山 528403

魏晓雅,女,研究实习员,主要从事病媒生物标本整理及序列分析,Email:weiyaya1990@163.com

收稿日期: 2016-04-05

网络出版日期: 2016-08-20

基金资助

国家科技支撑计划课题（2012BAK11B05）；广东省科技计划项目（2015A050502009）；国家质检总局项目（2015IK067，2015IK069）

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DNA barcoding and morphology identification of Pollenia rudis－a non-recorded fly species in China

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Zhongshan Entry-Exit Inspection and Quarantine Bureau Technology Center, Zhongshan 528403, Guangdong Province, China

Received date: 2016-04-05

Online published: 2016-08-20

Supported by

Supported by the National Science and Technology Support Program(No. 2012BAK11B05), Guangdong Province Science and Technology Program (No. 2015A050502009), and the Administration of Quality Supervision, Inspection and Quarantine Program of China(No. 2015IK067, 2015IK069)

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摘要

目的应用DNA条形码与形态分类相结合鉴定中山口岸入境船舶截获的雌性蝇类。方法 2015年3月26日，在中山小榄港口岸对截获的爱尔兰进口废旧瓦楞纸箱中的雌性蝇类进行DNA条形码与形态鉴定；与相似种伪粗野粉蝇进行形态与DNA条形码的比较。结果所截获的蝇类为雌性粗野粉蝇；粗野粉蝇与伪粗野粉蝇在形态上的区别较细微，而在658 bp的DNA条形码序列上有32个核苷酸的差异，差异高达4.86%，远大于DNA条形码2%的种判断界限。结论应用DNA条形码与形态分类相结合方法可以准确快速鉴定蝇类。

关键词： 雌性蝇类; 粗野粉蝇; 伪粗野粉蝇; DNA条形码和形态鉴定

本文引用格式

魏晓雅, 邱德义, 岳巧云, 刘德星 . 粗野粉蝇的DNA条形码和形态鉴定[J]. 中国媒介生物学及控制杂志, 2016 , 27(4) : 326 -329 . DOI: 10.11853/j.issn.1003.8280.2016.04.003

Abstract

Objective To identify the female fly intercepted from an entry ship rapidly and accurately with ‘Barcoding and Morphology’ (B & M)techniques. Methods Identifying the female fly which were intercepted at Zhongshan Xiaolan port on an entry ship from Ireland with waste paper on March 26 of 2015 by morphology and DNA barcoding, and comparing with its similar species Pollenia pediculate. Results The intercepted fly was P. rudis ♀. The differences between P. rudis and P. pediculate are subtle in morphology, but with 32 nucleotides differences in the 658 bp DNA barcoding fragment. The difference between these two species is up to 4.86%, far above 2%, the normal accepted species boundary with DNA barcoding. Conclusion The ‘B & M’ technique is a good tool to identify the female flies, especially the alien ones when no references are available.

Key words： Female fly; Pollenia rudis; Pollenia pediculate; DNA barcoding and morphology identification

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