河南口岸截获鼠类DNA条形码鉴定

张勤, 王晓梦, 岳巧云, 高洁, 邱德义

doi:10.11853/j.issn.1003.8280.2016.03.016

中国媒介生物学及控制杂志 >

2016 , Vol. 27 >Issue 3: 280 - 282

DOI: https://doi.org/10.11853/j.issn.1003.8280.2016.03.016

论著

河南口岸截获鼠类DNA条形码鉴定

展开

1 河南出入境检验检疫局技术中心医学媒介生物实验室, 郑州 450003;
2 中山出入境检验检疫局, 广东中山 528403

张勤,女,副主任技师,主要从事医学媒介生物条形码鉴定及其携带病原体检测,Email:qinzhang523@126.com

收稿日期: 2015-12-17

网络出版日期: 2016-06-20

收起

Identification of intercepted Rattus nitidus by DNA barcoding at Henan port

Expand

1 Henan Entry-Exit Inspection and Quarantine Bureau, Zhengzhou 450003, Henan Province, China;
2 Zhongshan Entry-Exit Inspection and Quarantine Bureau

Received date: 2015-12-17

Online published: 2016-06-20

Fold

摘要

目的利用DNA条形码技术对河南机场口岸截获的鼠类样本进行鉴定。方法提取该鼠类样本基因组DNA,以通用引物BatL5310和R6036R进行细胞色素C氧化酶亚基Ⅰ(COⅠ)基因片段的扩增并测序,将测序结果与GenBank及BOLD Systems v3中的序列进行比对,应用Mega 4.1软件构建系统发育树。结果该鼠样本COⅠ基因扩增片段去除引物后为702 bp,序列比对结果及系统进化关系表明其与大足鼠的同源性为99.43%,该鼠样本为大足鼠。结论 DNA条形码技术在鼠类鉴定中可弥补形态学鉴定的缺陷,是一种简单有效的鼠类鉴定方法。

关键词： DNA条形码; 分子鉴定; 大足鼠

本文引用格式

张勤, 王晓梦, 岳巧云, 高洁, 邱德义 . 河南口岸截获鼠类DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2016 , 27(3) : 280 -282 . DOI: 10.11853/j.issn.1003.8280.2016.03.016

Abstract

Objective DNA barcoding based on COⅠ gene was used to identify an unknown rat species captured at Henan port. Methods Genomic DNA of the unknown rat species was extracted, the international primer BatL5310 and R6036R were used to amplify COⅠ gene. Purified PCR product was sequenced and blasted in the GenBank and BOLD Systems v3. Phylogenetic trees were built with Mega 4.1. Results The amplified COⅠ fragment, without primer, of the unknown rat species was 750 bp in size and the sequence was 99.43% identical to the published sequence of Rattus nitidus. The unknown rat species was identified as R. nitidus. Conclusion DNA barcoding is a simple and effective method for the identification of rat species and can be the alternative method of morphological identification.

Key words： DNA barcoding; Molecular identification; Rattus nitidus

参考文献

[1] Hebert PDN, Cywinska A, Ball SL, et al. Biological identifications through DNA barcodes[J]. Proc R Soc Lond B Biol Sci,2003,270(1512):313-321.
[2] 吴薇. DNA条形码技术在医学媒介生物鉴定中的应用前景展望[J]. 畜牧与饲料科学,2012,33(1):22-24.
[3] 王颖,方绍庆,刘明杰,等. DNA条形码技术在媒介蝇类分子鉴定中的应用[J]. 口岸卫生控制,2013,18(3):55-58.
[4] 方义亮,张建庆,肖武,等. DNA条形码技术在麻蝇亚科鉴定中的应用[J]. 中国人兽共患病学报,2015,31(6):569-573.
[5] 刘德星,岳巧云,廖俊蕾,等. 国内未见分布蝇种:澳洲麻蝇的形态和DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2015,26(3):282-285.
[6] Yue QY, Wu KL,Qiu DY, et al. A formal re-description of the Cockroach Hebardina concinna anchored on DNA barcodes confirms wing polymorphism and identifies morphological characters for field identification[J]. PLoS One,2014,9(9):e106789.
[7] 刘德星,聂维忠,邱德义,等. 应用DNA条形码技术快速鉴定入境船舶上截获的昆虫蛹[J]. 检验检疫学刊,2014,24(5):53-57.
[8] Savolainen V, Cowan RS, Vogler AP, et al. Towards writing the encyclopaedia of life: an introduction to DNA barcoding[J]. Philos Trans R Soc Lond B Biol Sci,2005,360(1462): 1805-1811.
[9] Ward RD, Zemlak TS, Innes BH, et al. DNA barcoding Australia's fish species[J]. Philos Trans R Soc Lond B Biol Sci,2005,360(1462):1847-1857.
[10] Smith MA, Fisher BL, Hebert PDN. DNA barcoding for effective biodiversity assessment of a hyperdiverse arthropod group: the ants of Madagascar[J]. Philos Trans R Soc Lond B Biol Sci,2005,360(1462):1825-1834.
[11] Hebert PDN, Stoeckle MY, Zemlak TS, et al. Identification of birds through DNA barcodes[J]. PLoS Biol,2004,2(10):e312.
[12] Robins J, Hingston M, Matisoo-Smith E, et al. Identification Rattus species using mitochondrial DNA[J]. Mol Ecol Notes, 2007,7(5):717-729.
[13] 张际文. 中国国境口岸医学媒介生物鉴定图谱[M]. 天津:天津科学技术出版社,2015:63.
[14] 杨光荣,王应祥. 云南省啮齿动物名录及与疾病的关系[J]. 中国鼠类防制杂志,1989,5(4):222-229.
[15] Hebert PDN, Ratnasingham S, deWaard JR. Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species[J]. Proc Biol Sci,2003,270 Suppl 1:S96-99.
[16] 陈春生,张晓龙,张雪莲,等. 基于COⅠ基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用[J]. 西南国防医药, 2012,22(1):22-24.
[17] 闫东,鲁亮,金圣浩,等. DNA条形码技术在河北省鼠疫疫源地鼠种鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2014,25(1):21-23.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献