目的 对青海省1958－2013年从人体分离到的布鲁氏菌进行分析。方法 对1958年以来,临床诊断为布鲁氏菌病(布病)患者分离出的43株布鲁氏菌的来源、菌培养与临床关系等进行整理分析,硫堇和碱性复红的染料抑菌试验、单项血清凝集反应、噬菌体裂解试验对43株布鲁氏菌进行菌株表型分型鉴定。结果 取材337份做布鲁氏菌培养,分离出43株,其中慢性患者15例局部病灶检出布鲁氏菌。43株均为羊种布鲁氏菌,其中3株为羊1型,14株为羊2型,26株为羊3型。结论 羊种菌是青海省人群布病的致病菌。

Objective Isolated Brucella from human during 1958 to 2013 in Qinghai was analyzed. Methods Since 1958, the clinical diagnosis of brucellosis patients in bacteria from 43 strains of Brucella isolated culture were source and analyzed with clinical relationship, using the hydrogen sulfide produced determination, thionine and basic fuchsin dye bacteriostasis test, single serum agglutination reaction, phage splitting test on 43 strains of Brucella strains were phenotype subtype identification. Results From 337 for Brucella culture, isolated 43 strains of Brucella. The 15 patients with localized lesions were detected in chronic brucellosis. The 43 strains were 3 strains of Brucella melitensis, sheep, sheep 1 type 14 strains of type 2, 26 type 3 strains of sheep. Conclusion Sheep bacteria are pathogenic bacteria of brucellosis in Qinghai population.