长春市鼠类携带汉坦病毒的基因型分析

孙炳欣, 孙宇, 沈博, 吴静

doi:10.11853/j.issn.1003.4692.2015.06.016

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 6: 600 - 604

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.06.016

论著

长春市鼠类携带汉坦病毒的基因型分析

展开

1 长春市疾病预防控制中心病毒检验科, 长春 130033;
2 吉林省疾病预防控制中心

孙炳欣,女,硕士,检验技师,从事病毒检验工作,Email:63402608@qq.com

收稿日期: 2015-08-19

网络出版日期: 2015-12-20

收起

Genotype analysis of Hantavirus carried by the rodents in Changchun city

Expand

1 Changchun Center for Disease Control and Prevention, Changchun 130033, Jilin Province, China;
2 Jilin Center for Disease Control and Prevention

Received date: 2015-08-19

Online published: 2015-12-20

Fold

摘要

目的了解长春市鼠类动物所携带的汉坦病毒基因型别。方法采集长春市地区鼠肺样品,应用免疫荧光法检测鼠肺中汉坦病毒抗原,提取阳性样本中病毒RNA,利用RT-PCR法扩增S基因片段,并测序,利用DNAman软件和Mega 6.0 软件进行序列分析。结果在长春地区共捕获褐家鼠100 只,检测到阳性样本3 份,阳性率为3.0%。通过测序获得3株病毒的S基因片段序列,经同源性分析比较发现各序列间核苷酸同源性为100%,与其他汉城病毒(SEOV)核苷酸同源性在95.4%～100%之间,3株病毒全部为SEOV。系统发生树分析表明,3株病毒在同一组内,属于S3亚型。结论研究表明长春市褐家鼠中携带的汉坦病毒为SEOV型,S3亚型。

关键词： 汉坦病毒; 反转录-聚合酶链反应; 基因分型; 汉城病毒

本文引用格式

孙炳欣, 孙宇, 沈博, 吴静 . 长春市鼠类携带汉坦病毒的基因型分析[J]. 中国媒介生物学及控制杂志, 2015 , 26(6) : 600 -604 . DOI: 10.11853/j.issn.1003.4692.2015.06.016

Abstract

Objective To study the genotypes of the Hantavirus carried by the rodents in Changchun city. Methods Rat lung samples were collected in Changchun areas, and Hantavirus antigens in rat lungs were detected by immunofluorescence assay. The S gene fragment in HV-positive samples were amplified by RT-PCR and sequenced. The gene sequences were analyzed by using DNAman software and Mega 6.0 software. Results In this study, 100 Rattus norvegicus were captured in Changchun areas, of which 3 (3.0%) samples were positive for HV antigen. The S gene fragment of 3 strains were amplified from 3 positive samples. Homology analysis indicated that the nucleotide homology from the three S segment sequences was 100%, while that to other SEOV nucleotide homology was 95.4%-100%. All the 3 strains were SEOV. Phylogenesis analysis showed that the 3 strains were in the same group on the phylogenetic tree, which belonged to S3 sub-genotype. Conclusion The study shows that the Hantavirus carried by R. norvegicus in Changchun city was SEOV, the sub-genotype SEOV-3.

Key words： Hantavirus; Reverse transcription-polymerase chain reaction; Genotype; Seoul virus

参考文献

[1] Meyer BJ,Schmaljohn CS. Persistent hantavirus infections: characteristics and mechanisms[J]. Trends Microbiol,2000,8 (2):61-67.
[2] Elliott RM,Schmaljohn CS,Collett MS. Bunyaviridae genome structure and gene expression[J]. Curr Top Microbiol,1991, 169:91-141.
[3] Plyusnina A, Krajinovi? LC, Margaleti? J,et al. Genetic evidence for the presence of two distinct hantaviruses associated with apodemus mice in croatia and analysis of local strains[J]. J Med Virol,2011,83(1):108-114.
[4] Zhang YZ,Zou Y,Yan YZ,et al. Detection of phylogenetically distinct puumala-like viruses from red-grey vole Clethrionomys rufocanus in China[J]. J Med Virol,2007,79(8):1208-1218.
[5] Zhang YZ,Zou Y,Fu ZF,et al. Hantavirus infections in humans and animals,China[J]. Emerg Infect Dis,2010,16(8): 1195-1203.
[6] Jonsson CB, Figueiredo LT, Vapalahti O,et al. A global perspective on hantavirus ecology,epidemiology,and disease
[J]. Clin Microbiol Rev,2010,23(2):412-441.
[7] Khaibobllina SF,Morzunov SP,St Jeor SC. Hantaviruses: molecular biology,evolution and pathogenesis[J]. Curr Mol Med,2005,5(8):773-790.
[8] 邹洋,薛燕萍,陈化新,等. 汉坦病毒生物学特性研究进展[J]. 中华流行病学杂志,2006,27(9):819-822.
[9] 范胜涛,高晓龙,李元果,等. 吉林省汉坦病毒宿主动物携带病毒的遗传进化分析[J]. 中国生物制品学杂志,2014,27 (4):467-472.
[10] Plyusnin A. Genetics of hantaviruses implications to taxonomy
[J]. Arch Virol,2002,147(4):665-682.
[11] 李盈盈,杭长寿,刘旭,等. 聚合酶链式反应技术在肾综合征出血热病毒基因分型中的应用[J]. 中华实验和临床病毒学杂志,1994,8(1):20-25.
[12] 黄志成,濮小英,刘涛,等. 杭州地区汉坦病毒分离株的基因型分析[J]. 中国卫生检验杂志,2009,19(6):1225-1227.
[13] 吴东林,王慧,沈博,等. 吉林省普马拉病毒的RT-PCR检测与序列分析[J]. 中国卫生工程学,2008,7(5):259-261.
[14] Ahn C,Cho JT,Lee JG,et al. Detection of Hantaan and Seoul viruses by reverse transcriptase-polymerase chain reaction(RT- PCR)and restriction fragment length polymorphism(RFLP)in renal sysndrome patients with hemorrhagic fever[J]. Clin Nephrol,2000,53(2):79-89.
[15] Monroe MC,Morzanov SP,Johnson AM,et al. Genetic diversity and distribution of peromysus-borne hantaviruses in North America[J]. Emerg Infect Dis,1999,5(1):75-91.
[16] 王华,吉松组子,张全福,等. 汉城病毒新亚型的发现[J]. 中华实验和临床病毒学杂志,2000,14(4):317-321.
[17] 王平忠,白雪帆,黄长形. 汉滩病毒核衣壳蛋白C-端T细胞表位鉴定[J]. 中华微生物学和免疫学杂志,2004,24(5): 393-396.
[18] 解燕乡,杭长寿. 汉坦病毒与自然宿主动物共进化及实验动物模型的研究进展[J]. 中华实验和临床病毒学杂志,2000, 14(2):199-200,封三.
[19] 王世文,杭长寿,王华,等. 我国汉坦病毒基因型和基因亚型的分布研究[J]. 病毒学报,2002,18(3):211-216.
[20] 赵臣,赵阳,李艳,等. 吉林地区HFRS病原体基因型分析[J]. 微生物学杂志,2011,31(5):101-104.
[21] 李明慧,陈小萍,杨国庆,等. 葫芦岛市居民区褐家鼠携带的汉坦病毒分析[J]. 中国媒介生物学及控制杂志,2011,22 (3):239-242.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献