广东省蚊媒西尼罗病毒携带情况调查研究

段金花, 蔡松武, 吴军, 邹钦, 阴伟雄

doi:10.11853/j.issn.1003.4692.2015.06.005

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 6: 558 - 560

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.06.005

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广东省蚊媒西尼罗病毒携带情况调查研究

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广东省疾病预防控制中心消毒与病媒生物预防控制所, 广州 510300

段金花,女,副主任技师,主要从事病媒生物及其防制工作,Email:duankathie@163.com

收稿日期: 2015-09-07

网络出版日期: 2015-12-20

基金资助

广东省医学科学技术研究基金(A2013071)

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Investigation of West Nile virus carried by mosquito vectors in Guangdong province

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Guangdong Center for Disease Control and Prevention, Guangzhou 510300, Guangdong Province, China

Received date: 2015-09-07

Online published: 2015-12-20

Supported by

Supported by the Guangdong Medical Science and Technology Research Fund(No. A2013071)

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摘要

目的了解广东省蚊媒的构成、发生消长及西尼罗病毒的携带情况,为及时有效地切断西尼罗病毒病传播途径提供科学依据。方法 2013年5月至2014年4月在广东省的广州、佛山、东莞、茂名市的城区和郊区设点,采用灯诱法调查成蚊;一步法反转录-聚合酶链反应(RT-PCR)技术检测蚊媒携带的西尼罗病毒。结果广东省蚊媒平均密度为11.80只/灯,其密度高峰在4－5月,生境中以牲畜棚中的密度最高。致乏库蚊/致倦库蚊占捕蚊总数的89.90%,为优势蚊种。RT-PCR法未检测到西尼罗病毒阳性。结论致乏库蚊/致倦库蚊是广东省的优势蚊种,加强监测可以掌握其消长规律,为制定适宜的防制措施和预报预警其可能传播的西尼罗病毒提供科学依据;对广东省内西尼罗热的传播媒介种类及其传播流行风险有待进一步研究。

关键词： 西尼罗病毒; 蚊媒; 反转录-聚合酶链反应

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段金花, 蔡松武, 吴军, 邹钦, 阴伟雄 . 广东省蚊媒西尼罗病毒携带情况调查研究[J]. 中国媒介生物学及控制杂志, 2015 , 26(6) : 558 -560 . DOI: 10.11853/j.issn.1003.4692.2015.06.005

Abstract

Objective To study the community structure, dynamic and West Nile virus (WNV) carried of mosquito vectors in Guangdong, and provide scientific evidence for further development of control and prevention strategies. Methods Mosquito density was monitored by Light-trap from May 2013 to April 2014. One-step reverse-transcript PCR was applied. Results The average density of mosquito was 11.80/light. The seasonal peak of density was from May to July. Of all habitats the density in the barn was the highest. Culex pipiens quinquefasciatus accounts for 89.90%, the largest proportion of mosquitoes. No positive was found by RT-PCR. Conclusion Culex pipiens quinquefasciatus was the predominant species, the monitoring data could provide scientific basis for the control strategies and warning and forecasting of WNV, but the vectors species of West Nile fever in Guangdong province and its spread risk needs to be further studied.

Key words： West Nile virus; Mosquito vector; Reverse transcription-polymerase chain reaction

参考文献

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