目的 建立里海伊蚊的实验室饲养繁殖方法,了解克拉玛依地区里海伊蚊对常用杀虫剂的抗药性水平。方法 常规饲养里海伊蚊,对繁殖出的子一代采用幼蚊浸渍法,检测其对常用杀虫剂的半数致死浓度(LC₅₀)。结果 在室温(26±1)℃和相对湿度40%～60%的条件下,里海伊蚊卵孵化期为3 d,1～4龄幼虫期为7 d,从卵产下到羽化为11 d,一个卵荚孵化出110～120条幼虫。克拉玛依市敌敌畏、残杀威、溴氰菊酯、高效氯氰菊酯、双硫磷对里海伊蚊的LC₅₀分别为0.2838、2.7752、0.0024、0.0126和0.0033 mg/L。结论 克拉玛依地区里海伊蚊常用杀虫剂的LC₅₀本底值比已经报道过的白纹伊蚊要高。为了避免交互抗性的产生,该地区里海伊蚊的防治应采取以环境治理和生物防治为主、化学防治为辅的综合治理措施,注重统筹用药和农业合理规划,定期进行抗药性监测,做到科学合理用药。

Objective Establishment of Aedes caspius laboratory breeding methods, to evaluate the resistance in Ae. caspius to commonly used insecticides in Kalamay. Methods Conventional breeding Ae. caspius and determined via the dosing method and median lethal dose method to detection F1 generation. Results Under conditions of temperature of (26±1) ℃ and the relative humidity of (40-60) %, the time of egg was 3 d, the time of larva was 7 d, the time of egg to emergence was 11 d, an egg batch resulted in about 110-120 larvae (does Ae. capius lay eggs singly). The LC₅₀ values of DDVP, propoxur, deltamethrin, β-cypermethrin, and biothion to larvae of Ae. caspius were 0.2838,2.7752,0.0024,0.0126, and 0.0033 mg/L respectively. Conclusion The LC₅₀ values for commonly used insecticides to Ae. caspius is similar to Ae. albopictus. Breeding place should be administered first in controlling Ae. caspius and insecticides should be used properly to avoid resistance development.