新疆边境地区伊宁县图兰扇头蜱形态学及分子生物学鉴定

杜景云, 牟路萌, 张璘, 王丽娜, 王安东, 张科, 陈创夫, 王远志

doi:10.11853/j.issn.1003.4692.2015.03.013

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 3: 271 - 274

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.03.013

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新疆边境地区伊宁县图兰扇头蜱形态学及分子生物学鉴定

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1 新疆石河子大学医学院, 石河子832000;
2 山东省济南市中心医院;
3 新疆石河子大学动物科技学院;
4 河南省平顶山学院新农村发展研究院

杜景云,女,在读硕士,从事感染免疫学研究,Email: dujingyun025@163.com

收稿日期: 2014-12-15

网络出版日期: 2015-06-20

基金资助

国家973项目(2010CB530303);国家科技支撑项目(2013BAI05B05);国家自然科学基金(31060334)

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Morphological classification and molecular identification of Rhipicephalus turanicus in Yining county, Xinjiang Autonomous Region, China

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1 College of Medicine, Shihezi University, Shihezi 832000, Xinjiang Uygur Autonomous Region, China;
2 Blood Transfusion Dept of Jinan Central Hospital;
3 College of Animal Science, Shihezi University;
4 Research Institute of New Rural Development, Pingdingshan University

Received date: 2014-12-15

Online published: 2015-06-20

Supported by

Supported by the Grants from the International Cooperation in Science and Technology(No. 2010CB530303), the National Science and Technology Pillar Program(No. 2013BAI05B05), and National Natural Science Foundation of China(No. 31060334)

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摘要

目的对新疆边境地区伊宁县图兰扇头蜱进行形态学和分子生物学鉴定。方法在新疆边境地区的伊宁县随机采集畜牧体表寄生蜱324只,进行形态学初步筛选,从中选取6只形态差异较大的图兰扇头蜱,进行线粒体16S rRNA和线粒体细胞色素C氧化酶亚基Ⅰ(COⅠ)基因序列扩增、测序,并与GenBank登录的图兰扇头蜱参考序列进行遗传进化分析。结果形态学鉴定采集的伊宁县蜱类皆为图兰扇头蜱,两段序列的碱基组成均显示较高的A+T比例(16SrRNA 基因为73.8%,COⅠ基因为70.34%),在变异程度上16S rRNA 基因较COⅠ基因明显保守。结论首次应用形态学、16S rRNA和COⅠ基因测序分析表明新疆伊宁县采集蜱皆为图兰扇头蜱,并证明图兰扇头蜱具有生物多样性。

关键词： 图兰扇头蜱; 形态学结构; 分子鉴定; 16S rRNA基因; 线粒体细胞色素C氧化酶亚基Ⅰ基因

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杜景云, 牟路萌, 张璘, 王丽娜, 王安东, 张科, 陈创夫, 王远志 . 新疆边境地区伊宁县图兰扇头蜱形态学及分子生物学鉴定[J]. 中国媒介生物学及控制杂志, 2015 , 26(3) : 271 -274 . DOI: 10.11853/j.issn.1003.4692.2015.03.013

Abstract

Objective To identify Rhipicephalus turanicus in Yining county, Xinjiang Autonomous Region based on morphological and molecular evidence. Methods In total, 324 ticks were collected off livestock in the border areas of Yining county, Xinjiang Autonomous Region. All ticks collected were initially screened according to morphological features, and then 6 ticks were selected by morphological differences, then the 16S rRNA and COⅠ were amplified by PCR and subsequently sequenced, and phylogenetically analyzed with GenBank sequences. Results Rhipicephalus turanicus was observed and analyzed for its morphological characteristics. A high percentage of A+T base composition in the two sequences was observed, variation on the 16S rRNA gene was significantly conservative than COⅠ gene. Conclusion Rhipicephalus turanicus were firstly reported by morphological, 16S rRNA and COⅠ gene sequencing analysis, and it present high biodiversity in Yining county, Xinjiang.

Key words： Rhipicephalus turanicus; Morphological structure; Molecular identification; 16S rRNA; Cytochrome oxidase subunitⅠgene

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