秦皇岛口岸首例输入性登革热病例的分子溯源

杨鹏飞, 燕清丽, 张丽萍, 聂维忠, 甄维, 马雪征, 白红岩, 刘纯成, 时玉军, 陈跃, 孙肖红, 胡孔新

doi:10.11853/j.issn.1003.4692.2015.02.012

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 2: 155 - 158

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.02.012

论著

秦皇岛口岸首例输入性登革热病例的分子溯源

展开

1 淮安市疾病预防控制中心, 江苏淮安223001;
2 中国检验检疫科学研究院;
3 秦皇岛出入境检验检疫局

杨鹏飞,男,助理研究员,从事分子病毒学研究,Email:biomaster@126.com;燕清丽,女,助理研究员,从事分子病毒学研究,Email:yql0610@163.com

收稿日期: 2014-10-23

网络出版日期: 2015-04-20

基金资助

国家科技重大专项课题(2013ZX10004-101-006, 2013ZX10004805-008)

收起

Molecular origins of the first imported dengue fever case at frontier port in Qinhuangdao

Expand

1 Huaian Center for Disease Control and Prevention, Huaian 223001, Jiangsu Province, China;
2 Chinese Academy of Inspection and Quarantine;
3 Qinhuangdao Entry-Exit Inspection and Quarantine Bureau

Received date: 2014-10-23

Online published: 2015-04-20

Supported by

Supported by the Major National Science and Technology Projects for China (No. 2013ZX10004-101-006, 2013ZX10004805-008)

Fold

摘要

目的对2012年秦皇岛口岸的首例登革热输入性病例进行分子溯源, 确定病毒的基因型别及感染来源。方法应用ELISA方法和实时荧光RT-PCR方法对患者进行初筛, 利用RT-PCR法扩增病毒的NS1基因并测序, 根据获得的序列进行同源性分析、遗传距离计算及系统进化树的构建。结果从患者2份血清(QHD-01-BS1和QHD-01-BS2)中检测到IgM抗体阳性, 从QHD-01-BS1及尿液(QHD-01-US)中检测到登革热2型病毒的NS1基因。同源性分析发现QHD-01-BS1(US)与COM基因型中的印度株GWL39 INDI-01和GWL18 INDI-01同源性最高, 为97.3%;QHD-01-BS1(US)的遗传距离与COM基因型遗传距离最小, 为0.04;进化分析显示QHD-01-BS1(US)与GWL39 INDI-01和GWL18 INDI-01亲缘关系最近。结论分子溯源证实患者感染的登革热病毒与来源于印度的登革热2型病毒亲缘关系最近, 指示其传染源极有可能在印度。

关键词： 输入病例; 登革热病毒; 分子溯源; 基因分型

本文引用格式

杨鹏飞, 燕清丽, 张丽萍, 聂维忠, 甄维, 马雪征, 白红岩, 刘纯成, 时玉军, 陈跃, 孙肖红, 胡孔新 . 秦皇岛口岸首例输入性登革热病例的分子溯源[J]. 中国媒介生物学及控制杂志, 2015 , 26(2) : 155 -158 . DOI: 10.11853/j.issn.1003.4692.2015.02.012

Abstract

Objective To trace the virus from an imported viral hemorrhagic fever case in Qinhuangdao in 2012, molecular characteristics of the pathogen were studied. Methods The serum collected from the suspected case was detected for IgM antibody by ELISA, and viral nucleic acid of serum and urine samples were detected by real-time RT-PCR. Furthermore, NS1 segment was amplified by RT-PCR and sequenced. The genetic and phylogenetic analysis based on NS1 was computed. Results IgM anti-DENV was found in the serum QHD-01-BS1, BS2, and the NS1 gene sequences of serotype 2 of Dengue virus (DEN2) were recovered from the serum QHD-01-BS1 and the urine QHD-01-US respectively. The comparison showed the strains QHD-01-BS1 and QHD-01-US were very closely related to GWL39 INDI-01, GWL18 INDI-01 the COM genotype)isolated from India, with 97.3% sequence identity and 0.04 genetic distance. The further phylogenetic tree indicated the strains QHD-01-BS1 and QHD-01-US were classified as a lineage with GWL39 INDI-01, GWL18 INDI-01, belonged to genotype COM. Conclusion These results suggest the imported viral hemorrhagic fever case was infected by DENV2 from India and most likely infected in India.

Key words： Imported case; Dengue virus; Molecular origin; Genotype

参考文献

[1] Tuiskunen A, Monteil V, Plumet S, et al. Phenotypic and genotypic characterization of dengue virus isolates differentiates dengue feverand dengue hemorrhagic fever from dengue shock syndrome[J]. Arch Virol, 2011, 156(11):2023-2032.
[2] Lindenbach BD,Rice CM. Molecular biology offlaviviruses[J]. Adv Virus Res, 2003, 59(8):23-61.
[3] Bennett SN, Holmes EC, Chirivella M,et al. Molecular evolution of dengue 2 virus in Puerto Rico: positive selection in the viral envelope accompanies clade reintroduction[J]. J Gen Virol,2006, 87(4):885-893.
[4] WangE, NiHL, XuRL, etal. Evolutionaryrelationships of endemic/epidemic and sylvatic dengue viruses[J]. J Virol,2000, 74(7):3227-3234.
[5] 聂维忠, 李俊成, 李德昕, 等. 秦皇岛港入境国际航行船舶携带输入性医学媒介昆虫情况的调查[J]. 中国国境卫生检疫杂志, 2006, 29增刊1:85-87.
[6] 黄勇, 袁达康, 杨华可, 等. 广东省东莞市2010年首例输入性登革热病毒核酸检测及分析[J]. 中国卫生检验杂志, 2011,21(10):2450-2452.
[7] 王宇平, 张建庆, 许卿, 等. 福建口岸首次截获输入性登革热病例实验室诊断[J]. 中国国境卫生检疫杂志, 2010, 33(4): 237-240.
[8] 孔东锋, 张小岚, 谭唯. 2008年深圳市首起输入性登革热疫情分析[J]. 中国热带医学, 2008, 8(11):2013-2014.
[9] HirayamaaT,MizunocY,TakeshitacN, etal.Detectionof dengue virus genome in urine by real-time Reverse Transcriptase-PCR: a laboratory diagnostic method useful after disappearance of the genome in serum[J]. J Clin Microbiol, 2012, 50(6):2047-2052.
[10] Ma XZ,Zhen W, Yang PF,et al. First confirmation ofimported dengue virus serotype 2 complete genome in urine from a Chinese traveler returning from India[J]. Virol J, 2014, 11(4):56-61.
[11] KumarSRP, PatilJA, CeciliaD,etal. Evolution,dispersal and replacement of American genotype dengue type 2 viruses in India(1956-2005): selection pressure and molecular clock analyses[J]. J Gen Virol, 2010, 91(3):707-720.
[12] Shu PY,Su CL,Liao TL,et al. Molecular characterization of dengue viruses imported into Taiwan during 2003-2007: geographic distribution and genotype shift[J]. Am J Trop Med Hyg, 2009, 80(6):1039-1046.
[13] Bennett SN, Drummond AJ, Kapan DD, et al. Epidemic dynamics revealed in dengue evolution[J]. Mol Biol Evol, 2010, 27(4):811-818.
[14] Añez G,Morales-Betoulle ME,Rios M. Circulationofdifferent lineages of Dengue virus type 2 in central america,their evolutionary time-scale and selection pressure analysis[J]. PLoS One, 2011, 11(6):e27459.
[15] TwiddySS,FarrarJJ,VinhChauN,et al.Phylogenetic relationships and differential selection pressures among genotypes of Dengue-2 virus[J]. Virology, 2002, 298(1):63-72.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献