ISSN 1003-8280 CN 10-1522/R 中国疾病预防控制中心 主办
收稿日期: 2014-11-20
网络出版日期: 2015-04-20
基金资助
国家自然科学基金(81290342);国家重点基础研究发展计划(973计划)(2011CB504702)
Surveillance on mosquito-borne arboviruses in Xixian county and Xin'an county, Henan province, 2012
Received date: 2014-11-20
Online published: 2015-04-20
Supported by
Supported by the National Natural Science Foundation of China(No. 81290342)and the National Key Basic Research Program of China(973 Program)(No. 2011CB504702)
目的 了解河南省部分地区蚊传虫媒病毒的种类分布及基因型别。方法 于2012年5－8月在河南省洛阳市新安县和信阳市息县的居民住房、猪圈、牛棚及树林中采集蚊虫。使用细胞培养法分离病毒, 通过反转录-聚合酶链反应(RT-PCR)方法进行种属特异扩增鉴定, 并用Clustal X2.1、MegAlign、Genedoc 3.2和Mega v5.1生物信息学软件完成病毒核酸序列的分子生物学分析并进行基因分型。结果 共采集蚊虫4属5种7149只, 其中新安县以骚扰阿蚊居多(2055只, 51.36%), 息县以淡色库蚊(2964只, 94.16%)为主。共分离获得5株病毒, 直接接种BHK-21细胞无病变、C6/36细胞培养仅导致轻微病变, 其C6/36细胞传代培养物转接BHK-21细胞后3 d可致细胞明显病变;序列分析表明这5株病毒均属基因Ⅰ型流行性乙型脑炎病毒(JEV), 其中3株分离自淡色库蚊, 2株来自三带喙库蚊。结论 河南省部分地区依然存在JEV及其传播媒介, 且目前当地自然界中循环的JEV仍以基因Ⅰ型为主。
郑雅匀, 付士红, 唐晓燕, 李幸乐, 尚思远, 徐超, 梁国栋 . 河南省新安县和息县2012年蚊传虫媒病毒调查[J]. 中国媒介生物学及控制杂志, 2015 , 26(2) : 127 -132 . DOI: 10.11853/j.issn.1003.4692.2015.02.005
Objective To understand the species, distribution and genotype of mosquito-borne arboviruses in some regions of Henan province. Methods Mosquito samples were collected from Xixian county and Xin'an county in Henan province from May to August, 2012. After species identification, mosquitoes were inter-cells cross cultured for viral isolation. RT-PCR using specific primer for common arboviruses was used to identify the positive isolates. Molecular biological analysis were conducted by using software of Clustal X2.1, MegAlign, Genedoc 3.2 and Mega v5.1, and genotype for the virus was identified. Results A total of 7149 mosquitoes which belong to 5 species in 4 genera were collected. The predominant mosquito specie in Xin'an county was Armigeres subalbatus (2055, 51.36%), but the mainly species in Xixian county was Culex pipens pallens (2964, 94.16%). Five strains from Cx. pipens pallens and Cx. tritaeniorhynchus were identified as genotypeⅠ Japanese encephalitis virus (JEV), which caused slightly cytopathic effect (CPE) in C6/36 cells, and no CPE in BHK-21. But severe CPE was observed in BHK-21 when 3 days after inoculating the C6/36 culture of the viruses onto BHK-21 cells. Conclusion In Xixian county and Xin'an county of Henan province, b
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