首次在长角血蜱中检测到汉赛巴尔通体

吴海霞, 李志芳, 刘起勇, 张卫东, 栗冬梅, 马怀雷, 鲁亮, 刘京利

doi:10.11853/j.issn.1003.4692.2015.01.004

中国媒介生物学及控制杂志 >

2015 , Vol. 26 >Issue 1: 16 - 18

DOI: https://doi.org/10.11853/j.issn.1003.4692.2015.01.004

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首次在长角血蜱中检测到汉赛巴尔通体

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1. 中国疾病预防控制中心传染病预防控制所媒介生物控制室, 传染病预防控制国家重点实验室, 世界卫生组织媒介生物监测与管理合作中心, 北京102206;
2. 郑州大学公共卫生学院;
3. 郑州大学附属洛阳中心医院

吴海霞,女,博士,副研究员,主要从事蜱类相关研究,Email: wuhaixia@icdc.cn

收稿日期: 2014-09-25

网络出版日期: 2015-02-20

基金资助

国家自然科学基金(30600513);国家传染病科技重大专项(2012ZX10004-219)

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First detection of Bartonella henselae infection in Haemaphysalis longicornis

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1. WHO Collaborating Centre for Vector Surveillance and Management, State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China;
2. Department of Epidemiology and Health Statistics, College of Public Health, Zhengzhou University;
3. Luoyang Central Hospital Affiliated to Zhengzhou University

Received date: 2014-09-25

Online published: 2015-02-20

Supported by

Supported by the National Natural Science Foundation of China(No. 30600513)and the National Science and Technology Major Project for Infectious Diseases in China(No. 2012ZX10004-219)

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摘要

目的检测长角血蜱中汉赛巴尔通体的携带情况。方法将采获于石家庄市灵寿县的长角血蜱分组,经无菌处理后研磨匀浆,一部分直接提取DNA进行PCR检测,另一部分接种于含5%去纤维羊血的胰酶大豆培养基上,在37 ℃,5%CO₂的培养箱中进行培养后,挑取疑似菌落进行PCR检测。对所得阳性条带的PCR产物测序,并将所测核酸序列在GenBank中进行序列比对。结果菌落提取的DNA样品中有2个样品出现阳性条带,但测序未得结果;直接提取的DNA样品中有1个样品出现阳性条带,经过同源性比对后为汉赛巴尔通体。结论石家庄市灵寿县采集到的长角血蜱中存在汉赛巴尔通体感染,这是首次在长角血蜱中检测到汉赛巴尔通体。

关键词： 长角血蜱; 汉赛巴尔通体; 分离培养; 聚合酶链反应

本文引用格式

吴海霞, 李志芳, 刘起勇, 张卫东, 栗冬梅, 马怀雷, 鲁亮, 刘京利 . 首次在长角血蜱中检测到汉赛巴尔通体[J]. 中国媒介生物学及控制杂志, 2015 , 26(1) : 16 -18 . DOI: 10.11853/j.issn.1003.4692.2015.01.004

Abstract

Objective To detect the infection with Bartonella henselae in Haemaphysalis longicornis. Methods Haemaphysalis longicornis captured in Lingshou county, Shijiazhuang, Hebei province, China, were divided into groups. The H. longicornis in each group was disinfected, sterilized, and grinded. Half of the grinded mixture was directly used for DNA extraction and polymerase chain reaction (PCR) analysis;the other half was inoculated onto tryptic soy agar supplemented with 5% defibrinated sheep blood followed by culturing in a 5% CO₂ incubator at 37 ℃, and the suspected colonies were selected for PCR analysis. The PCR products that produced positive bands were sequenced, and the nucleotide sequences obtained were aligned with known sequences in GenBank. Results A positive band was observed in one sample from direct DNA extraction, the sequence of which was found to belong to B. henselae by alignment analysis. Positive bands were observed in two DNA samples isolated from bacterial cultures, but no results were produced from sequencing. Conclusion Haemaphysalis longicornis collected in Lingshou county, Shijiazhuang, are infected with B. henselae. This is the first detection of B. henselae infection in H. longicornis.

Key words： Haemaphysalis longicornis; Bartonella henselae; Culture; Polymerase chain reaction

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