专家论坛

应用沃尔巴克氏体通过种群替换阻断蚊媒病的传播

展开
  • 1. 中山大学-密歇根州立大学热带病虫媒控制联合研究中心, 广东广州510080;
    2. 中国疾病预防控制中心传染病预防控制所, 传染病预防控制国家重点实验室;
    3. 世界卫生组织媒介生物监测与管理合作中心;
    4. 美国密歇根州立大学微生物学和分子遗传学系
李永军,男,博士,从事感染沃尔巴克氏体的蚊虫宿主的媒介效能研究,Email: liyongjun_sysu@126.com

收稿日期: 2014-09-18

  网络出版日期: 2015-02-20

基金资助

教育部科学技术研究重点(重大)项目(311030);广东省引进创新科研团队计划(2011S009);广州市开发区科技领军人才项目(2013L-P116);美国国立卫生研究院和盖茨全球健康大挑战联合基金;美国国立卫生研究院/国家过敏症与传染病研究所基金(R01 AI-080597);国际原子能地区合作项目(RAS5066)

Blocking the transmission of mosquito-borne diseases through Wolbachia-based population replacement

Expand
  • 1. Sun Yat-sen University-Michigan State University Joint Center of Vector Control for Tropical Diseases, Key Laboratory for Tropical Disease Control, Ministry of Education, Guangzhou 510080, Guangdong Province, China;
    2. State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention;
    3. WHO Collaborating Centre for Vector Surveillance and Management;
    4. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA

Received date: 2014-09-18

  Online published: 2015-02-20

Supported by

Supported by the Key Project of Chinese Ministry of Education(No. 311030), Guangdong Innovative Research Team Program (No. 2011S009), Scientific and Technological Leading Talents of Guangzhou Development District (No. 2013L-P116), a Grant from the Foundation for the NIH Through the Grand Challenges in Global Health Initiative of the Bill, Melinda Gates Foundation, a Grant(No. R01 AI-080597)and from the National Institutes of Health/National Institute for Allergy and Infectious Disease and the IAEA-TC Project(No. RAS5066)

摘要

由蚊类传播的疾病每年造成人类大量的病例和死亡,然而包括登革热一类的重要蚊媒病至今尚无有效的疫苗用于预防。由于传统的、依赖化学杀虫剂的蚊媒控制方法正面临不断增长的昆虫抗药性和对环境的污染问题,探索对蚊媒的可持续性防治手段正成为目前研究的热点。沃尔巴克氏体可以导致宿主产生胞质不相容现象,并能诱导蚊媒产生对多种人类病原体的抗性,因此基于沃尔巴克氏体的蚊媒种群替换在理论上可以阻断蚊媒病传播。相比于其他生物防治方法,该方法具有低成本、可持续以及环保等特点,已在多个国家开展实地测试,它也是目前最具潜力的蚊媒病生物防治手段之一。

本文引用格式

李永军, 刘起勇, 奚志勇 . 应用沃尔巴克氏体通过种群替换阻断蚊媒病的传播[J]. 中国媒介生物学及控制杂志, 2015 , 26(1) : 11 -15 . DOI: 10.11853/j.issn.1003.4692.2015.01.003

Abstract

Diseases transmitted by mosquitoes result in severe morbidity and mortality every year, and there is an urgent need to develop an effective tool to prevent the most important diseases including dengue and others. Due to rapidly developing resistance in insects and its negative impact on environment, chemical insecticide becomes increasingly difficult to be relied on for disease control. Thus a significant effort is made to develop a sustainable vector control strategy, such as Wolbachia-based vector control. Theoretically Wolbachia-based population replacement would lead to interruption of disease transmission due to the ability of Wolbachia to induce cytoplasmic incompatibility and pathogen interference. Compared with the other control tools, this Wolbachia approach is cost-effective, sustainable and environmental friendly. With field trial currently ongoing in a number of countries, Wolbachia-based vector control is now becoming one of the most promising novel tools for control of mosquito-borne diseases.

参考文献

[1] World Health Organization. Dengue and severe dengue[Z]. http://www.who.int/mediacentre/factsheets/fs117/en/.
[2] Alonso PL,Brown G,Arevalo-Herrera M,et al. A research agenda to underpin malaria eradication[J]. PLoS Med,2011,8: e1000406.
[3] Norrby R. Outlook for a dengue vaccine[J]. Clin Microbiol Infec, 2014,20(S5):92-94.
[4] Daily JP. Malaria vaccine trials-beyond efficacy end points[J]. New Engl J Med,2012,367(24):2349-2351.
[5] Farenhorst M,Mouatcho JC,Kikankie CK,et al. Fungal infection counters insecticide resistance in African malaria mosquitoes[J]. Proc Natl Acad Sci USA,2009,106(41):17443-17447.
[6] Kanzok SM, Jacobs-Lorena M. Entomopathogenic fungi as biological insecticides to control malaria[J]. Trends Parasitol, 2006,22(2):49-51.
[7] Fuchs S,Nolan T,Crisanti A. Mosquito transgenic technologies to reduce Plasmodium transmission[J]. Methods Mol Biol,2013, 923:601-622.
[8] Sumitani M,Kasashima K,Yamamoto DS,et al. Reduction of malaria transmission by transgenic mosquitoes expressing an antisporozoite antibody in their salivary glands[J]. Insect Mol Biol,2013,22(1):41-51.
[9] Wang S,Ghosh AK,Bongio N,et al. Fighting malaria with engineered symbiotic bacteria from vector mosquitoes[J]. Proc Natl Acad Sci USA,2012,109(31):12734-12739.
[10] Alphey L,Benedict M,Bellini R,et al. Sterile-insect methods for control of mosquito-borne diseases:an analysis[J]. Vector-Borne Zoonotic Dis,2010,10(3):295-311.
[11] Wimmer EA. Applications of insect transgenesis[J]. Nat Rev Genet,2003,4(3):225-232.
[12] 潘晓玲,刘起勇,奚志勇. 基于昆虫共生菌沃尔巴克氏体的蚊 媒和蚊媒病控制研究进展[J]. 中国媒介生物学及控制杂志, 2014,25(1):1-7.
[13] 郑小英,刘起勇,奚志勇. 基于沃尔巴克氏体的蚊媒和蚊媒病 控制的生物安全性[J]. 中国媒介生物学及控制杂志,2014, 25(2):93-96.
[14] Hertig M, Wolbach SB. Studies on rickettsia-like micro-organisms in insects[J]. J Med Res,1924,44(3):329-374.
[15] Yen JH, Barr AR. The etiological agent of cytoplasmic incompatibility in Culex pipiens[J]. J Invertebr Pathol,1973,22 (2):242-250.
[16] Hoffmann AA,Montgomery BL,Popovici J,et al. Successful establishment of Wolbachia in Aedes populations to suppress dengue transmission[J]. Nature,2011,476(7361):454-457.
[17] Xi ZY,Khoo CCH,Dobson SL. Wolbachia establishment and invasion in an Aedes aegypti laboratory population[J]. Science, 2005,310(5746):326-328.
[18] Bian G,Joshi D,Dong Y,et al. Wolbachia invades Anopheles stephensi populations and induces refractoriness to Plasmodium infection[J]. Science,2013,340(6133):748-751.
[19] Walker T,Johnson PH,Moreira LA,et al. The wMel Wolbachia strain blocks dengue and invades caged Aedes aegypti populations[J]. Nature,2011,476(7361):450-453.
[20] Hedges LM,Brownlie JC,O'Neill SL,et al. Wolbachia and virus protection in insects[J]. Science,2008,322(5902):702.
[21] Teixeira L,Ferreira Á,Ashburner M. The bacterial symbiont Wolbachia induces resistance to RNA viral infections in Drosophila melanogaster [J]. PLoS Biol,2008,6 (12) : e1000002.
[22] Panteleev DY,Goryacheva II, Andrianov BV, et al. The endosymbiotic bacterium Wolbachia enhances the nonspecific resistance to insect pathogens and alters behavior of Drosophila melanogaster[J]. Russian J Genet,2007,43(9):1066-1069.
[23] Bian GW,Zhou GL,Lu PZ,et al. Replacing a native Wolbachia with a novel strain results in an increase in endosymbiont load and resistance to dengue virus in a mosquito vector[J]. PLoS Negl Trop Dis,2013,7(6):e2250.
[24] Blagrove MSC,Arias-Goeta C,Di Genua C,et al. A Wolbachia wMel transinfection in Aedes albopictus is not detrimental to host fitness and inhibits Chikungunya virus[J]. PLoS Negl Trop Dis, 2013,7(3):e2152.
[25] Blagrove MSC,Arias-Goeta C,Failloux AB,et al. Wolbachia strain wMel induces cytoplasmic incompatibility and blocks dengue transmission in Aedes albopictus[J]. Proc Natl Acad Sci USA,2012,109(1):255-260.
[26] Moreira LA,Iturbe-Ormaetxe I,Jeffery JA,et al. A Wolbachia symbiont in Aedes aegypti limits infection with dengue, Chikungunya, and Plasmodium[J]. Cell,2009,139(7):1268-1278.
[27] Glaser RL,Meola MA. The native Wolbachia endosymbionts of Drosophila melanogaster and Culex quinquefasciatus increase host resistance to West Nile virus infection[J]. PLoS One,2010, 5(8):e11977.
[28] Xi ZY,Dean JL,Khoo C,et al. Generation of a novel Wolbachia infection in Aedes albopictus (Asian tiger mosquito) via embryonic microinjection[J]. Insect Biochem Mol Biol,2005,35 (8):903-910.
[29] Xi ZY, Khoo CCH, Dobson SL. Interspecific transfer of Wolbachia into the mosquito disease vector Aedes albopictus[J]. Proc Biol Sci,2006,273(1592):1317-1322.
[30] Mcmeniman CJ,Lane RV,Cass BN,et al. Stable introduction of a life-shortening Wolbachia infection into the mosquito Aedes aegypti[J]. Science,2009,323(5910):141-144.
[31] Fu Y,Gavotte L,Mercer DR,et al. Artificial triple Wolbachia infection in Aedes albopictus yields a new pattern of unidirectional cytoplasmic incompatibility[J]. Appl Environ Microbiol,2010,76(17):5887-5891.
[32] Cook PE, Mcmeniman CJ, O'Neill SL. Modifying insect population age structure to control vector-borne disease[J]. Adv Exp Med Biol,2008,627:126-140.
[33] Min KT,Benzer S. Wolbachia,normally a symbiont of Drosophila, can be virulent,causing degeneration and early death[J]. Proc Natl Acad Sci USA,1997,94(20):10792-10796.
[34] Moreira LA,Saig E,Turley AP,et al. Human probing behavior of Aedes aegypti when infected with a life-shortening strain of Wolbachia[J]. PLoS Negl Trop Dis,2009,3(12):e568.
[35] Evans O, Caragata EP, Mcmeniman CJ, et al. Increased locomotor activity and metabolism of Aedes aegypti infected with a life-shortening strain of Wolbachia pipientis[J]. J Exp Biol, 2009,212(10):1436-1441.
[36] Turley AP,Moreira LA,O'Neill SL,et al. Wolbachia infection reduces blood-feeding success in the dengue fever mosquito, Aedes aegypti[J]. PLoS Negl Trop Dis,2009,3(9):e516.
[37] Mcmeniman CJ, Hughes GL, O'Neill SL. A Wolbachia symbiont in Aedes aegypti disrupts mosquito egg development to a greater extent when mosquitoes feed on nonhuman versus human blood[J]. J Med Entomol,2011,48(1):76-84.
[38] Mcmeniman CJ,O'Neill SL. A virulent Wolbachia infection decreases the viability of the dengue vector Aedes aegypti during periods of embryonic quiescence[J]. PLoS Negl Trop Dis,2010, 4(7):e748.
[39] Eliminate dengue program[Z]. http://www.eliminatedengue.com.
[40] Bian GW,Xu Y,Lu P,et al. The endosymbiotic bacterium Wolbachia induces resistance to Dengue virus in Aedes aegypti[J]. PLoS Pathog,2010,6(4):e1000833.
[41] Turley AP,Zalucki MP,O'Neill SL,et al. Transinfected Wolbachia have minimal effects on male reproductive success in Aedes aegypti[J]. Parasit Vectors,2013,6:36.
[42] Zhang GM,Hussain M,O'Neill SL,et al. Wolbachia uses a host microRNA to regulate transcripts of a methyltransferase, contributing to Dengue virus inhibition in Aedes aegypti[J]. Proc Natl Acad Sci USA,2013,110(25):10276-10281.
[43] Rancès E,Johnson TK,Popovici J,et al. The Toll and Imd pathways are not required for Wolbachia-mediated Dengue virus interference[J]. J Virol,2013,87(21):11945-11949.
[44] Caragata EP,Rancès E,Hedges LM,et al. Dietary cholesterol modulates pathogen blocking by Wolbachia[J]. PLoS Pathog, 2013,9(6):e1003459.
[45] Pan XL,Zhou GL,Wu JH,et al. Wolbachia induces reactive oxygen species(ROS)-dependent activation of the Toll pathway to control Dengue virus in the mosquito Aedes aegypti[J]. Proc Natl Acad Sci USA,2012,109(1):E23-E31.
[46] Perera R,Riley C,Isaac G,et al. Dengue virus infection perturbs lipid homeostasis in infected mosquito cells[J]. PLoS Pathog, 2012,8(3):e1002584.
[47] Lu P,Bian GW,Pan XL,et al. Wolbachia induces density-dependent inhibition to Dengue virus in mosquito cells[J]. PLoS Negl Trop Dis,2012,6(7):e1754.
[48] Rancès E,Yixin HY,Woolfit M,et al. The relative importance of innate immune priming in Wolbachia-mediated dengue interference[J]. PLoS Pathog,2012,8(2):e1002548.
[49] Wong ZS,Hedges LM,Brownlie JC,et al. Wolbachia-mediated antibacterial protection and immune gene regulation in Drosophila[J]. PLoS One,2011,6(9):e25430.
[50] Hussain M,Frentiu FD,Moreira LA,et al. Wolbachia uses host microRNAs to manipulate host gene expression and facilitate colonization of the dengue vector Aedes aegypti[J]. Proc Natl Acad Sci USA,2011,108(22):9250-9255.
[51] Bourtzis K,Pettigrew MM,O'Neill SL. Wolbachia neither induces nor suppresses transcripts encoding antimicrobial peptides[J]. Insect Mol Biol,2000,9(6):635-639.
[52] Lu P,Bian GW,Pan XL,et al. Wolbachia induces density-dependent inhibition to Dengue virus in mosquito cells[J]. PLoS Negl Trop Dis,2012,6(7):e1754.
[53] Osborne SE,Leong YS,O'Neill SL,et al. Variation in antiviral protection mediated by different Wolbachia strains in Drosophila simulans[J]. PLoS Pathog,2009,5(11):e1000656.
[54] Osborne SE,Iturbe-Ormaetxe I,Brownlie JC,et al. Antiviral protection and the importance of Wolbachia density and tissue tropism in Drosophila simulans[J]. Appl Environ Microbiol, 2012,78(19):6922-6929.
[55] Micieli MV,Glaser RL. Somatic Wolbachia (Rickettsiales: Rickettsiaceae) levels in Culex quinquefasciatus and Culex pipiens(Diptera:Culicidae)and resistance to West Nile virus infection[J]. J Med Entomol,2014,51(1):189-199.
[56] Frentiu FD,Zakir T,Walker T,et al. Limited Dengue virus replication in field-collected Aedes aegypti mosquitoes infected with Wolbachia[J]. PLoS Negl Trop Dis,2014,8(2): e2688.
[57] Mcgraw EA,Merritt DJ,Droller JN,et al. Wolbachia density and virulence attenuation after transfer into a novel host[J]. Proc Natl Acad Sci USA,2002,99(5):2918-2923.
[58] Sinkins SP. Wolbachia and arbovirus inhibition in mosquitoes[J]. Future Microbiol,2013,8(10):1249-1256.
[59] Hancock PA, Sinkins SP, Godfray HCJ. Strategies for introducing Wolbachia to reduce transmission of mosquito-borne diseases[J]. PLoS Negl Trop Dis,2011,5(4):e1024.

文章导航

/