新截获中国未见分布种杜比亚丽蝇形态和DNA条形码鉴定

廖俊蕾,岳巧云,邱德义,魏晓雅,刘德星,贾凤龙

doi:10.11853/j.issn.1003.4692.2014.06.006

中国媒介生物学及控制杂志 >

2014 , Vol. 25 >Issue 6: 509 - 513

DOI: https://doi.org/10.11853/j.issn.1003.4692.2014.06.006

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新截获中国未见分布种杜比亚丽蝇形态和DNA条形码鉴定

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1 中山大学生命科学学院,广东广州 510275; 2 中山出入境检验检疫局,广东中山 528403

廖俊蕾,女,在读硕士,主要从事蝇科及丽蝇科的形态学分类鉴定

收稿日期: 2014-08-04

网络出版日期: 2014-12-20

基金资助

国家科技支撑计划课题(2012BAK11B05); 国家质检总局计划项目(2012IK223); 广东出入境检验检疫局计划项目(2013GDK36, 2013GDK39); 中山市科技项目(20123A298)

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Morphology and DNA barcoding of a newly intercepted fly species exoticin China, Calliphora dubia (Macquart, 1855)

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1 School of Life Sciences, Sun Yat?Sen University, Guangzhou 510275, Guangdong Province, China;2 Zhongshan Entry-Exit Inspection and Quarantine Bureau, Zhongshan 528403, Guangdong Province, China

Received date: 2014-08-04

Online published: 2014-12-20

Supported by

Supported by the National Key Technology R & D Program （No. 2012BAK11B05）, Administration of Quality Supervision,Inspection and Quarantine Science and Technology Project （No. 2012IK223）, Guangdong Entry?Exit Inspection and Quarantine Bureau Science and Technology Project （No. 2013GDK36, 2013GDK39） and the Zhongshan City Science and Technology Project （No. 20123A298）

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摘要

目的　利用DNA条形码技术鉴定外来媒介生物,提高物种鉴定的速度与准确度。方法　对2013年9月在中山神湾口岸截获来自澳大利亚的丽蝇进行形态与DNA条形码鉴定,通过与澳大利亚的蝇类专家进行标本交流,进一步确认鉴定结果;并与相似种螳丽蝇进行形态与DNA条形码上的比较。结果　通过形态观察与DNA条形码鉴定,所截获的丽蝇为杜比亚丽蝇;经科技查新,确认为中国未见分布种。结论　DNA条形码是传统形态分类的有力补充,对在国境卫生检验检疫工作中及时鉴定媒介生物,减少媒介生物本身及其携带病原体的扩散,减少对公共卫生的安全威胁起到重要作用。

关键词： 杜比亚丽蝇; DNA条形码和形态鉴定; 中国未见分布种

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廖俊蕾,岳巧云,邱德义,魏晓雅,刘德星,贾凤龙 . 新截获中国未见分布种杜比亚丽蝇形态和DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2014 , 25(6) : 509 -513 . DOI: 10.11853/j.issn.1003.4692.2014.06.006

Abstract

Objective　To apply DNA barcoding in species identification of exotic vectors. Methods　Morphology and DNA barcoding based identification was performed on two blowflies from Australia that were intercepted at the Shenwan port (Zhongshan, Guangdong) in September 2013. The identification results were confirmed with specialists in Australia and compared to data of a similar species, Calliphora augur. Results　The two blowflies were identified as C. dubia, which represents exotic species in China based on sci?tech novelty search. Conclusion　DNA barcoding is a strong supplementary tool for conventional taxonomy, which plays an important role in frontier health and quarantine inspection by timely identifying vector insects, reducing the spread of vector insects and the carried pathogens, and mitigating threats to public health.

Key words： Calliphora dubia; DNA barcoding and morphological identification; Exotics species in China

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