调查研究

一例输入性板齿鼠的种类鉴定与分析

展开
  • 大榭出入境检验检疫局,浙江 宁波 315812
胡群,男,硕士,高级工程师,主要从事口岸医学媒介生物与病原微生物检测研究,Email: nbhuqun@sina.com

收稿日期: 2014-08-12

  网络出版日期: 2014-10-20

Species identification of an imported rodent sample of Bandicota indicaby morphological and genetic analysis

Expand
  • Daxie Exit-Entry Inspection and Quarantine Bureau, Ningbo 315812, Zhejiang Provinca, China

Received date: 2014-08-12

  Online published: 2014-10-20

摘要

目的 对1份从入境集装箱中检获的鼠样品进行种类鉴定。方法 运用形态学方法及DNA条形码技术进行鉴定分析。结果 鼠样品体长约252 mm,尾长220 mm,后足长40 mm,毛色呈黑褐色, cytB基因序列与板齿鼠同源性最为接近,与不同板齿鼠的遗传距离为0.048~0.175。结论 经形态鉴定和DNA条形码比对,确定为板齿鼠,系浙江省口岸首次检获该鼠种,为加强外来医学媒介生物防控提供了基础依据。

本文引用格式

胡群, 马思杰, 马研, 梅勇 . 一例输入性板齿鼠的种类鉴定与分析[J]. 中国媒介生物学及控制杂志, 2014 , 25(5) : 464 -465 . DOI: 10.11853/j.issn.1003.4692.2014.05.022

Abstract

Objective To identify the species of a rodent sample captured in an imported container at the port of Zhejiang province, China. Methods Morphological methods and DNA barcoding were applied for rodent identification. Results The rodent had dark-brown fur with an approximate body length of 252 mm, a tail length of 220 mm, and a hind foot length of 40 mm. Genetic analysis of the mitochondrial cytB gene showed that the rodent shared the highest sequence homology with Bandicota indica and matched different individuals at a genetic distance of 0.048 to 0.175. Conclusion Based on morphological characterization and DNA barcode comparison, the imported rodent sample was identified as B. indica. This species was captured for the first time at the port of Zhejiang province, which provided a reference to exclude invasion of exotic disease vectors.

参考文献

[1] 王健胜,高雅琴. 基于cytB基因的动物检材种属鉴定在非传统领域的部分运用[J]. 生物技术通报,2010(1):37-43.
[2] 王晓梅,杨秀荣. DNA分子标记研究进展[J]. 天津农学院学报, 2000,7(1):21-24.
[3] Hebert PDN, Cywinska A, Ball SL, et al. Biological identifications through DNA barcodes[J]. Pro Bio Sci,2003,270(1512):313-321.
[4] Castro JA, Picornell A, Ramon M. Mitochondrial DNA: a tool for populational genetics studies[J]. Internat Microbiol,1998,1:327-332.
[5] 牛李丽,李岩,古小彬,等. 基于cytB基因对大熊猫等6种珍稀野生动物感染蛔虫的种系发育分析[J]. 中国兽医科学,2012,42(9):895-899.
[6] Nicolas V, Quérouil S, Verheyen E, et al. Mitochondrial phylogeny of African wood mice, genus Hylomysus (Rodentia, Muridae): Implications for their taxonomy and biogeography[J]. Mol Phylogenet Evol,2006,38(3):779-793.
[7] 郑剑宁,裘炯良. 东南亚七国常见害鼠种类及其分布概况[J]. 中国国境卫生检疫杂志,2008,31(4):265-267.
[8] 龚震宇,侯娟,傅桂明,等. 浙江省2010年鼠类种群密度与季节消长监测分析[J]. 中国媒介生物学及控制杂志,2012,23(3):244-248.
[9] 何国华,易波,朱光峰,等. 宁波市鼠类种群及其分布调查[J]. 中国媒介生物学及控制杂志,2002,13(1):56-57.
[10] 黄文几. 中国啮齿类[M]. 上海:复旦大学出版社,1995:164-166.
文章导航

/