目的 了解登封市屠宰场生猪小肠结肠炎耶尔森菌带菌率、血清分型、毒力基因分布等情况,为制定预防措施提供参考依据。方法 从屠宰场生猪扁桃体及回盲部内容物中分离小肠结肠炎耶尔森菌,并进行生化鉴定、血清分型、毒力基因PCR检测。结果 从196份生猪咽拭子中分离出107株小肠结肠炎耶尔森菌,检出率为54.59%;从196份生猪肛拭子中分离出36株小肠结肠炎耶尔森菌,检出率为18.37%。检出菌血清型为O∶3,生物型为3型,Ⅰ型(ail＋、ystA＋、ystB－、yadA＋、virF＋)占93.71%(134/143),Ⅱ型(ail＋、ystA＋、ystB－、yadA－、virF－)占6.29%(9/143)。结论 登封市生猪小肠结肠炎耶尔森菌以致病性菌株为主,今后应加强该菌的进一步监测工作。
Objective To investigate the detection rate, serotype, and virulence gene distribution of Yersinia enterocolitica isolated from live pigs in Dengfeng, China, and to provide reference for the development of preventive measures. Methods Yersinia enterocolitica was isolated from the pharyngeal swabs (tonsils) and intestinal contents (at the ileocecal junction), which were collected from the live pigs in abattoirs in Dengfeng. Biochemical identification, serotyping, and PCR detection of virulence gene were performed on the isolates. Results A total of 107 strains of Y. enterocolitica were isolated from 196 pharyngeal swabs, with a detection rate of 54.59%; 36 strains were isolated from 196 intestinal contents, with a detection rate of 18.37%. The serotype of the isolates was O∶3. Three biological types were identified as follows: typeⅠ (ail＋, ystA＋, ystB－, yadA＋, virF＋), with a ratio of 93.71% (134/143), and typeⅡ (ail＋, ystA＋, ystB－, yadA－, virF－), with a ratio of 6.29% (9/143). Conclusion The pathogenic strains are dominant among Y. enterocolitica isolated from live pigs in Dengfeng. Monitoring of Y. enterocolitica should be strengthened in the future.
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