目的 调查二连浩特口岸鼠体寄生蜱种群组成及自然感染病原体情况。方法 从捕获的鼠体上收集寄生蜱, 提取单只蜱DNA基因组及总RNA, 采用PCR或RT?PCR方法检测莱姆病螺旋体、Q热立克次体、巴贝西原虫、斑点热立克次体、无形体、森林脑炎病毒、布尼亚病毒、克里米亚-刚果出血热病毒。结果 共采集鼠体寄生蜱152只, 分别为草原血蜱、银盾革蜱和亚东璃眼蜱, 其中草原血蜱为优势种, 占捕获总数的98.03%。从草原血蜱、银盾革蜱和亚东璃眼蜱中检测到斑点热立克次体19份, 检出率为12.50%, 其他病原体检测均为阴性。结论 二连浩特口岸地区存在斑点热立克次体自然疫源地。
曹晓梅, 张晓龙, 房魏, 乔舜, 柳文进, 杨鹏飞, 魏怀波, 田丽, 姚李四, 郭天宇, 杨宇
. 二连浩特口岸鼠体寄生蜱感染病原体调查[J]. 中国媒介生物学及控制杂志, 2014
, 25(2)
: 127
-130
.
DOI: 10.11853/j.issn.1003.4692.2014.02.010
Objective To investigate the species of ticks parasitizing wild rodents and tick?borne pathogens at Erenhot port. Methods Ticks were collected from captured field rats, and genomic DNA and total RNA were extracted from each tick. PCR and RT?PCR were used to detect Borrelia burgdorferi, Q fever rickettsia, Babesia microti, spotted fever group rickettsia (SFGR), Anaplasma, Russian spring?summer Encephalitis virus, Bunyavirus, and Crimean?Congo hemorrhagic fever virus. Results A total of 152 parasitic ticks were collected, including Haemaphysalis verticalis, Dermacentor niveus, and Hyalomma asiaticum, and H. verticalis was the dominant species, accounting for 98.03% of all captured ticks. Nineteen cases of SFGR were detected among H. verticalis, D. niveus, and H. asiaticum, with a detection rate of 12.50%, but the other seven species of pathogens were not detected. Conclusion There are natural foci of SFGR in the area of Erenhot port.
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