目的 了解广东省蚊虫种群中沃尔巴克氏体(Wolbachia)的分布及其基因型别。方法 利用Wolbachia的wsp基因序列建立PCR检测方法, 对蚊科中库蚊属、伊蚊属、阿蚊属和按蚊属部分蚊虫种类进行检测, 同时利用Mega 5.2软件对所获基因序列进行系统分析。结果 分别从致倦库蚊、骚扰阿蚊、白纹伊蚊及未分型蚊种中检测到12条目的基因序列, 其中11条成功进行测序, 这些序列分别属于A和B 2个超级基因组, 分布在5个不同的基因族中。结论 广东省白纹伊蚊、骚扰阿蚊和致倦库蚊中均存在A大组(含2个小组)和B大组(含3个小组)基因型的Wolbachia感染, 埃及伊蚊和中华按蚊未检测出Wolbachia。
Objective To understand Wolbachia distribution and genotype among mosquito populations in Guangdong. Methods PCRs were performed on mosquitoes from Culex, Aedes, Anopheles and Armigeres based on Wolbachia wsp gene, PCR products were sequenced and performed phylogenetic analysis using molecular biology software (Mega 5.2). Results Twelve samples were positive on Cx. pipiens quinquefasciatus, Ar. subalbatus, Ae. albopictus and unidentified Culex, 11 of them were successfully sequenced, phylogenetic analysis revealed they were divided into 5 clades belonged to Wolbachia supergroup A and supergroup B. Conclusion Both supergroup A and supergroup B were detected among Cx. pipiens quinquefasciatus, Ar. subalbatus, Ae. albopictus, but not in Ae. aegypti and Anopheles sinensis samples.
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