目的 调查浙江省啮齿动物巴尔通体携带状况,为巴尔通体人群感染的预防控制提供科学依据。方法 用夹夜法在浙江省不同地区、不同季节捕获啮齿动物,无菌操作取啮齿动物的肝和脾,用聚合酶链反应(PCR)检测巴尔通体,然后对部分阳性产物测序,将所测核酸序列提交到GenBank,做相似性比较及序列分析。结果 从黑线姬鼠、褐家鼠、小家鼠、东方田鼠、北社鼠和黄毛鼠中检测到巴尔通体特异DNA片段,阳性率分别为5.76%(17/295)、2.86%(1/35)、10.00%(1/10)、5.26%(1/19)、3.91%(7/179)和3.03%(1/33),浙江省首次从小家鼠中检测到巴尔通体DNA片段;岱山、海宁、建德、丽水、仙居、龙游地区的阳性率分别为2.63%(3/114)、10.84%(9/83)、2.88%(3/104)、11.11%(12/108)、0(0/46)和0.57%(1/175);4个季度阳性率分别为3.39%(4/118)、6.08%(9/148)、2.95%(7/237)、6.30%(8/127),平均阳性率为4.44%(28/630);遗传进化分析提示检测到的巴尔通体与Bartonella henselae遗传关系最接近。结论 浙江省啮齿动物中广泛存在巴尔通体感染,存在对人群致病的风险,应采取措施加以控制。
Objective To investigate the Bartonella infection in rodents in Zhejiang province, China and to provide baseline data for the control and prevention of Bartonella infection in humans. Methods Rodents were captured by night trapping in different areas and seasons in Zhejiang province, and their liver and spleen were aseptically collected to detect Bartonella by PCR. Some positive amplicons were sequenced and the nucleotide sequences tested were submitted to GenBank. Similarity comparison and sequence analysis were then performed to identify the Bartonella species. Results The specific DNA fragments of Bartonella were detected in Apodemus agrarius (5.76%, 17/295), Rattus norvegicus (2.86%, 1/35), Mus musculus (10.00%, 1/10), Microtus fortis (5.26%, 1/19), Niviventer confucianus (3.91%, 7/179), and Rattus losea (3.03%, 1/33). Furthermore, Bartonella DNA fragments were detected from M. musculus for the first time in Zhejiang. The positive rates were 2.63% (3/114), 10.84% (9/83), 2.88% (3/104), 11.11% (12/108), 0% (0/46), and 0.57% (1/175) in Daishan, Haining, Jiande, Lishui, Xianju, and Longyou, respectively. The positive rates were 3.39% (4/118), 6.08% (9/148), 2.95% (7/237), and 6.30% (8/127) in the four seasons, respectively. The average positive rate was 4.44% (28/630). Phylogenetic analysis suggested that the detected Bartonella had the closest genetic relationship with Bartonella henselae. Conclusion The prevalence of Bartonella infection in rodents in Zhejiang province has posed a risk of transmission to humans, and corresponding control measures should be taken into consideration.
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