目的 了解中华按蚊对溴氰菊酯抗药性现状,为合理使用杀虫剂提供科学依据。方法 采用WHO的成蚊滤纸接触法,观察首只蚊虫击倒时间、击倒率和死亡率,以区分剂量判定抗性级别。结果 江陵县、襄州区、枣阳市、通城县、天门市、夷陵区、武穴市、广水市、曾都区、京山县和恩施市被溴氰菊酯击倒的首只中华按蚊时间分别为6、20、11、9、20、6、30、5、14、25和23 min,60 min时的击倒率(%)分别为9.62、7.69、14.55、2.11、3.00、48.94、2.80、10.78、14.00、1.00和3.06; 11个地区中华按蚊在溴氰菊酯区分剂量作用下的死亡率除恩施市为70.85%外,其余均＜50.00%,为高抗群体。 结论 湖北区域范围内中华按蚊对溴氰菊酯的抗击倒力强,抗性程度高,抗性发展快,应加强监测,现场应用宜适当加大使用剂量,确保防制效果。
Objective To investigate the resistance of Anopheles sinensis to deltamethrin and provide baseline data for proper use of insecticide. Methods The filter paper contact method for adult mosquitoes, as recommended by WHO, was used to determine the time for initial knockdown, knockdown rate, and mortality of An. sinensis, and the levels of resistance were determined based on the discriminating doses. Results For the An. sinensis in Jiangling county, Xiangzhou district, Zaoyang city, Tongcheng county, Tianmen city, Yiling district, Wuxue city, Guangshui city, Zengdu district, Jingshan county, and Enshi city, the time for initial knockdownby deltamethrin was 6, 20, 11, 9, 20, 6, 30, 5, 14, 25, and 23 min, respectively; the knockdown rate caused by deltamethrin at 60 min was 9.62%, 7.69%, 14.55%, 2.11%, 3.00%, 48.94%, 2.80%, 10.78%, 14.00%, 1.00%, and 3.06%, respectively. The mortality of An. sinensis at the discriminating dose of deltamethrin was less than 50.00% in all sampling areas except for Enshi where the mortality was 70.85%. Conclusion The resistance of An. sinensis to deltamethrin in Hubei province was high and the spreading of resistance was rapid. Thus, we should strengthen the monitoring of insecticide resistance and implement strategic dosing of insecticide to achieve better control effect.
[1] 李玉贵,孔祥骊,李振海,等. 河北省康保鼠疫自然疫源地动物流行病学调查分析[J]. 中国媒介生物学及控制杂志,1992,3(2):95-98.
[2] 刘满福,刘合智,张彩虹. 河北省鼠疫自然疫源地内啮齿动物种类及其分布的调查[J]. 中国媒介生物学及控制杂志,2002,13(4):260-262
[3] Witt JDS, Therloff DL, Hebert PDN. DNA barcoding reveals extraordinary cryptic diversity in an amphipod genus: implications for desert spring conservation[J]. Mol Ecol,2006,15(10): 3073-3082.
[4] Hebert PDN, Ratnasingham S,deWaard JR. Barcoding animal life: cytochrome Coxidase subunit 1 divergences among closely related species[J]. Proc Royal Soci London,Series B,Bio Sci,2003,270 Suppl 1:S96-99.
[5] 马英. DNA条形码技术在青海省小型兽类及寄生蚤鉴定中的应用研究[D]. 北京:中国疾病预防控制中心,2010.
[6] 郑智民,姜志宽,陈安国. 啮齿动物学[M]. 上海:上海交通大学出版社,2008:57-74.
[7] 程佳月,王丽华,彭克美,等. 国际生命条形码计划:DNA Barcoding[J]. 中国畜牧兽医,2009,36(8):49-53.
[8] 孟玮,杨天燕,海萨,等. 基于线粒体COⅠ基因序列的亚东鲑DNA条形码研究[J]. 水产学杂志,2010,23(1):6-10.
[9] 陈春生,张晓龙,张雪莲,等. 基于COⅠ基因序列的DNA条形码在中尼边境鼠类物种鉴定中的应用[J]. 西南国防医药,2012, 22(1):22-24.
[10] 马英,鲁亮. DNA条形码技术研究新进展[J]. 中国媒介生物学及控制杂志,2010,21(3):275-280.
[11] Nolan DV,Dallas JF,Mordue Luntz AJ. Molecular taxonomy and population structure of a Culicoides midge vector[J]. Vet Ital,2004,40(3):352-359.
[12] 吴薇. DNA条码技术在医学媒介生物鉴定中的应用前景展望[J]. 畜牧与饲料科学,2012,33(1):22-24.
