目的 对青海省蚊虫标本中新分离的QH07130病毒株进行系统鉴定分析。方法 采用血清学和分子生物学方法对QH07130病毒进行鉴定,并对该病毒进行系统进化特征分析。结果 QH07130病毒株对C6/36细胞产生明显细胞病变;间接免疫荧光试验显示,QH07130病毒株与辽宁病毒(LNV)单克隆抗体呈现阳性反应。聚丙烯酰胺凝胶电泳显示,QH07130病毒为12条带双链RNA病毒,与LNV的6-5-1带型一致。该病毒第10节段序列测定全长为844 bp,与LNV SX0771和NE9712株核苷酸同源性分别为99.5%和98.0%;系统进化分析显示,QH07130病毒位于LNV进化分支内,并且与LNV SX0771和NE9712株的进化关系最近,确认QH07130病毒为LNV。结论 青海省蚊虫中存在LNV传播,这是首次在位于青藏高原的青海省分离到该病毒。

Objective To identify the arbovirus strain QH07130 isolated from Culex modestus collected in Qinghai province, China. Methods The virus QH07130 was identified by serological and molecular biological methods, and phylogenetic analysis of this virus was performed. Results QH07130 propagated in C6/36 cells and caused significant cytopathic effects. As shown by indirect immunofluorescence assay (IFA), QH07130 reacted with the monoclonal antibodies against Liaoning virus (LNV). Polyacrylamide gel electrophoresis showed that QH07130 was a virus with 12 double-stranded RNA segments, which was in accordance with the 6-5-1 banding pattern of LNV. Sequence analysis of the 10th segment of QH07130 revealed that the sequence length was 844 bp, and its nucleotide homologies with LNV SX0771 and NE9712 amounted 99.5% and 98.0%, respectively. Phylogenetic analysis demonstrated that QH07130 was located in the same evolutionary branch with LNV and had the closest revolutionary relationship with LNV SX0771 and NE9712. QH07130 was identified as LNV. Conclusion LNV is transmitted by mosquitoes in Qinghai province. And QH07130 is the first strain of LNV isolated in Qinghai province.