中国媒介生物学及控制杂志 >

2013 , Vol. 24 >Issue 5: 382 - 388

DOI: https://doi.org/10.11853/j.issn.1003.4692.2013.05.002

论著

基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)

展开
  • 第二军医大学热带传染病学教研室, 上海 200433
马颖(1983- ),女,动物学硕士,主要从事媒介生物学研究工作。Email: m_y_1001@yahoo.cn

收稿日期: 2013-05-20

  网络出版日期: 2013-10-20

基金资助

国家自然科学基金委员会-云南省人民政府联合基金(U0932604)

收起

Phylogenetic relationship among some species of genus Anopheles subgenus Anopheles (Diptera：Culicidae) in China：based on rDNA-ITS2 sequences

Expand
  • Department of Tropical Infectious Diseases, Second Military Medical University, Shanghai 200433, China

Received date: 2013-05-20

  Online published: 2013-10-20

Supported by

Supported by the National Natural Science Foundation of China-Yunnan Joint Fund (No. U0932604)

Fold

摘要

目的 阐明中国按蚊属按蚊亚属蚊种的亲缘关系。方法 根据核糖体DNA第2内转录间隔区(rDNA-ITS2)序列,采用邻接法(NJ)、最大简约法(MP)、最小进化法(ME)和最大似然法(ML),对采自中国的按蚊亚属22种(分子型)蚊虫进行系统发育关系重建。结果 rDNA-ITS2序列的长度范围为340～1754 bp,种内的p遗传距离均小于0.004,种间为0.009～0.420,平均值为0.224。各种方法重建的系统发育树均显示分为3大支,分别对应赫坎按蚊种团和须喙按蚊种团各一支,以及林氏按蚊与五斑按蚊种团聚为的一支,三者为并系关系;赫坎按蚊种团成员种分为主要的4支,其种间关系与形态分类结果一致,但分子型spLL1和spLL2的聚类位置不稳定。结论 重建的分子系统发育树客观地阐明了中国按蚊属按蚊亚属各种团及蚊种之间的亲缘关系,ITS2序列具有足够的信息贡献量。

关键词： 按蚊属; 按蚊亚属; 核糖体DNA第2内转录间隔区; 系统发育

本文引用格式

马颖, 马雅军, 林琳, 王琰 . 基于rDNA-ITS2序列的中国按蚊属按蚊亚属部分种类的系统发育关系研究(双翅目：蚊科)[J]. 中国媒介生物学及控制杂志, 2013 , 24(5) : 382 -388 . DOI: 10.11853/j.issn.1003.4692.2013.05.002

Abstract

Objective To clarify the interspecific phylogenetic relationship among the species of genus Anopheles subgenus Anopheles in China. Methods The phylogenetic relationship among 22 species (molecular forms) of subgenus Anopheles collected from China was reconstructed based on ribosomal DNA second internal transcribed spacer (rDNA-ITS2) sequences using neighbour joining, maximum parsimony, minimum evolution and maximum likelihood methods. Results The length range of rDNA-ITS2 was 340-1754 bp; the intraspecific genetic distances (p distance) were below 0.004, and the range of pairwise genetic distances among Anopheles mosquitoes was 0.009-0.420 (mean 0.224). All the reconstructed phylogeny trees showed that there were three clades, i.e., An. hyrcanus group, An. barbirostris group and An. lindesayi with An. maculipennis group and that the group clades were paraphyletic. There were four major clusters in An. hyrcanus group, and the interspecific relationship in each cluster was the same as the morphological taxonomy. However, the cluster positions of spLL1 and spLL2 were unstable. Conclusion The molecular phylogenetic tree reconstructed in this study objectively shows the interspecific phylogenetic relationship among the groups and species of genus Anopheles subgenus Anopheles in China. The rDNA-ITS2 sequences have sufficient contribution to the reconstruction of phylogeny of Anopheles species.

Key words： Genus Anopheles; Subgenus Anopheles; Ribosomal DNA second internal transcribed spacer; Phylogeny

参考文献


[1] 陆宝麟,许锦江,俞渊,等. 中国动物志. 昆虫纲. 第9卷. 双翅目:蚊科(下卷)
[M]. 北京:科学出版社,1997:1-62.

[2] Harbach RE, Howard TM. Index of currently recognized mosquito species (Diptera: Culicidae)
[J]. Eur Mosq Bull,2007,23:1-66.

[3] Harbach RE. Mosquito taxonomic inventory
[EB/OL].
[2012-09-28]Anopheles classification. http://mosquito-taxonomic-inventory.info/anopheles-classification.

[4] Mohanty A, Swain S, Kar SK, et al. Analysis of the phylogenetic relationship of Anopheles species, subgenus Cellia (Diptera: Culicidae) and using it to define the relationship of morphologically similar species
[J]. Infect Genet Evol,2009,9(6):1204-1224.

[5] Dixit J, Srivastava H, Sharma M, et al. Phylogenetic inference of Indian malaria vectors from multilocus DNA sequences
[J]. Infect Genet Evol,2010,10(6):755-763.

[6] Bourke BP, Foster PG, Bergo ES, et al. Phylogenetic relationships among species of Anopheles (Nyssorhynchus) (Diptera: Culicidae) based on nuclear and mitochondrial gene sequences
[J]. Acta Trop, 2010,114(2):88-96.

[7] Krzywinski J, Grushko OG, Besansky NJ. Analysis of the complete mitochondrial DNA from Anopheles funestus: an improved dipterans mitochondrial genome annotation and a temporal dimension of mosquito evolution
[J]. Mol Phylogenet Evol,2006,39(2): 417-423.

[8] Logue K, Chan ER, Phipps T, et al. Mitochondrial genome sequences reveal deep divergences among Anopheles punctulatus sibling species in Papua New Guinea
[J]. Malaria J,2013,12:64.

[9] Ambrose L, Riginos C, Cooper RD, et al. Population structure, mitochondrial polyphyly and the repeated loss of human biting ability in anopheline mosquitoes from the southwest Pacific
[J]. Mol Ecol,2012,21:4327-4343.

[10] 吴静,马雅军,马颖. 基于mtDNA和rDNA基因序列的中国按蚊属塞蚊亚属种类的系统发育研究
[J]. 昆虫学报,2011,53(9): 1030-1038.

[11] 马雅军,吴静,马颖. 基于rDNA-ITS2序列的中国按蚊属塞蚊亚属部分种类的系统发育研究
[J]. 昆虫分类学报,2011,33(4): 245-256.

[12] 马颖,马雅军,孟祥梅,等. 我国辽宁省赫坎按蚊种团的分子鉴别研究
[J]. 寄生虫与医学昆虫学报,2009,16(4):224-227.

[13] Collins FH, Mendez MA, Rasmussen MO, et al. A ribosomal RNA gene probe differentiates member species of the Anopheles gambiae complex
[J]. Am J Trop Med Hyg,1987,37(1):37-41.

[14] Porter CH, Collins FH. Species diagnostic differences in a ribosomal DNA internal transcribed spacer from the sibling species Anopheles freeborni and Anopheles hermsi (Diptera: Culicidae)
[J]. Am J Trop Med Hyg,1991,45(2):271-279.

[15] 伍桐,马雅军. rDNA-ITS2序列作为中国按蚊分子鉴别特征的评述:Ⅰ.按蚊亚属
[J]. 寄生虫与医学昆虫学报,2013,20(1): 16-24.

[16] Harbach RE. The classification of genus Anopheles (Diptera: Culicidae): a working hypothesis of phylogenetic relationships
[J]. Bull Entomol Res,2004,946:537-553.

[17] Foley DH, Bryan JH, Yeates D. Evolution and systematics of Anopheles: insights from a molecular phylogeny of Australasian mosquitoes
[J]. Mol Phylogenet Evol,1998,9(2):262-275.

[18] Sallum MAM, Schultz TR, Wilkerson RC. Phylogeny of Anophelinae (Diptera: Culicidae) based on morphological characters
[J]. Ann Entomol Soc Am,2000,93(4):745-775.

[19] Harbach RE, Kitching LJ. Reconsideration of anopheline mosquito phylogeny (Diptera: Culicidae: Anophelinae) based on morphological data
[J]. Syst Biodivers,2005,3(4):345-374.

[20] Park MH, Choochote W, Junkum A, et al. Reproductive isolations of Anopheles sinensis from Anopheles lesteri and Anopheles sineroides in Korea
[J]. Genes Genomics,2008,30(3):245-252.

[21] Ma YJ, Xu JN. The Hyrcanus group of Anopheles (Anopheles) in China (Diptera: Culicidae): species discrimination and phylogenetic relationships inferred by ribosomal DNA internal transcribed spacer 2 sequences
[J]. J Med Entomol,2005,42(4): 610-619.

[22] 陈锡欣,李石柱,邓绪礼,等. 山东省赫坎按蚊复合体成员种的分子鉴别研究
[J]. 中国寄生虫病防治杂志,2003,16(4):232-234.

[23] 林琳,杨曼尼,南春燕,等. 中国部分地区赫坎按蚊种团的分子鉴别研究
[J]. 中国媒介生物学及控制杂志,2013,24(2):93-98.

[24] 马雅军,瞿逢伊. 我国赫坎按蚊复合体成员种的rDNA-ITS2区序列差异及系统发育分析
[J]. 昆虫知识,2002,39(3):209-214.

[25] Hwang UW. Revisited ITS2 phylogeny of Anopheles (Anopheles) hyrcanus group mosquitoes: reexamination of unidentified and misidentified ITS2 sequences
[J]. Parasitol Res,2007,101: 885-894.

[26] Paredes-Esquivel C, Harbach RE,Townson H. Molecular taxonomy of members of the Anopheles hyrcanus group from Thailand and Indonesia
[J]. Med Vet Entomol,2011,25:348-352.

[27] Harrison BA, Scanlon JE. The subgenus Anopheles in Thailand (Diptera: Culicidae)
[J]. Contr Am Entomol Inst,1975,12:1-307.

[28] Paredes-Esquivel C, Donnelly MJ, Harbach RE, et al. A molecular phylogeny of mosquitoes in the Anopheles barbirostris subg