中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (2): 102-104.

• 论著 • 上一篇    下一篇

白纹伊蚊3日龄成蚊饱血前后唾液腺总蛋白谱

吴家红孙宇程金芝陈璐   

  1. 贵阳医学院寄生虫学教研室 / 现代病原生物学基础实验室 (贵阳 550004)
  • 收稿日期:2008-10-27 出版日期:2009-04-20 发布日期:2009-04-20
  • 作者简介:吴家红(1974-),女,博士,副教授,主要从事医学昆虫防制与虫媒病毒研究。
  • 基金资助:

    国家自然科学基金(3001015); 贵州省省长基金资助

Total protein spectrum of salivary gland from Aedes albopictus aged 3 days after a blood meal

 WU Jia-Hong, SUN Yu, CHENG Jin-Zhi, CHEN Lu   

  1. Department of Parasitology / Basic Laboratory of Modern Pathogenic Biology, Guiyang Medical College, Guiyang, Guizhou 550004, China
  • Received:2008-10-27 Online:2009-04-20 Published:2009-04-20

摘要:

  【摘要】 目的 探讨3日龄白纹伊蚊雌蚊吸食糖水组和饱血组唾液腺总蛋白谱的差异。方法 分别采用Pierce蛋白定量试剂盒和SDS?PAGE电泳分析白纹伊蚊3日龄吸食糖水组和饱血组成蚊唾液腺蛋白质浓度和蛋白谱。结果 糖水组蚊虫单对唾液腺蛋白质质量约为(1.243±0.460)μg,饱血组约为(0.945±0.460)μg;SDS?PAGE检测到糖水组唾液腺总蛋白有12条主带,糖水组与饱血组唾液腺总蛋白条带没有变化。结论 白纹伊蚊雌蚊饱血后唾液腺总蛋白无特异条带诱导,但饱血后单对唾液腺总蛋白下降。

关键词: 白纹伊蚊, 唾液腺, SDS?PAGE

Abstract:

  【Abstract】 Objective To detect the difference of the salivary protein spectrum from Aedes albopictus aged 3 days with blood-feed and sugar-feed, respectively.  Methods The  salivary  protein was detected with Pierce BCA Protein Assay Kit and SDS-PAGE. Results The amount of salivary gland proteins in sugar-feed and blood-feed mosquitoes aged 3 days was approximately (1.243±0.460)μg/female and (0.945±0.460)μg/female, respectively.  There were at least twelve major proteins in sugar-feed mosquito salivary glands analyzed by SDS-PAGE, and no difference was detected in the electrophoretic bands between sugar-feed and blood-feed mosquitoes salivary gland. Conclusion No specific protein was induced after Ae.albopictus feeding blood, but the total protein of salivary gland per female decreased after a blood meal.

Key words: Aedes albopictus, Salivary gland, SDS-PAGE

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