中国媒介生物学及控制杂志 ›› 2009, Vol. 20 ›› Issue (4): 349-351.

• 论著 • 上一篇    下一篇

2007年浙江省鼠类感染汉坦病毒监测及病毒分离

姚苹苹1,徐芳1,朱函坪1,谢荣辉1,程胤凯1,梅玲玲1,朱智勇1,邓小昭2,张云2,王忠灿2   

  1. 1 浙江省疾病预防控制中心  浙江省肾综合征出血热疫苗研究重点实验室(杭州 310051); 2 南京军区军事医学研究所
  • 收稿日期:2009-02-17 出版日期:2009-08-20 发布日期:2009-08-20
  • 作者简介:姚苹苹(1967-),女,浙江台州人,副主任技师,从事汉坦病毒研究。
  • 基金资助:

    国家“863”高技术研发计划(2007AA02Z465); 全军“十一五”科技攻关项目(06G65); 南京军区医药卫生(08MB151)

Surveillance on natural infection of rodents with Hantavirus in Zhejiang province and isolation of Hantavirus

YAO Ping-Ping, XU Fang, ZHU Han-Ping, XIE Rong-Hui, CHENG Yin-Kai, MEI Ling-Ling, ZHU Zhi-Yong, DENG Xiao-Zhao, ZHANG Yun, WANG Zhong-Can   

  1. Zhejiang Center for Disease Control and Prevention, Hangzhou, Zhejiang 310051, China
  • Received:2009-02-17 Online:2009-08-20 Published:2009-08-20

摘要:

  【摘要】 目的 了解浙江省鼠类自然感染汉坦病毒(HV)情况和病毒型别,为肾综合征出血热(HFRS)防治提供科学依据。方法 采集鼠类肺组织和血清标本,用间接免疫荧光试验检测鼠血清IgG抗体,直接免疫荧光试验检测鼠肺HV抗原。HV抗原阳性鼠肺接种Vero?E6细胞分离病毒,用HV单克隆荧光抗体鉴定分离株的型别。结果 共捕获鼠形动物1129只,其中黑线姬鼠为优势种,鼠肺HV抗原阳性率为3.0%,鼠血标本IgG抗体阳性57份,阳性率8.0%。分离到6株汉滩(HTN)型病毒,其中来自黑线姬鼠5株,褐家鼠1株。结论 浙江省存在以黑线姬鼠为主要传染源的HFRS疫源地,HV主要流行型别为HTN型。

关键词: 啮齿动物, 汉坦病毒, 监测

Abstract:

  【Abstract】 Objective To clarify the natural infection situation of rodents by Hantavirus (HV) and HV strains in Zhejiang province in 2007,and provide science evidence for the prevention and control of hemorrhagic fever with renal syndrome (HFRS).Methods Lung tissue and serum from rodent were sampled, and IgG antibody from serum was tested by indirect immunofluorscence assay and direct immunofluorscence assay was adopted to test HV antigens from lung tissues. HV was isolated by Vero?E6 cells, and HV strains were identified by Anti?McAb.Results A total of 1129 rodents were captured in 2007.The dominant specie was Apodemus agrarius, and the positive rate of HV antigen in rodent lungs was 3.0%. The IgG antibody of 57 blood samples was positive, and the positive rate was 8.0%.  Six  strains  of  hantaan (HTN)  virus  were  isolated,  five  strains  from A.agrarius  and  one  from  Rattus norvegicus.  Conclusion  There  are  natural  foci  of  HFRS  which  main  infection  sources  are A.agrarius in Zhejiang province, and HTN strain could be the main prevalence strains of HV.

Key words: Rodent, Hantavirus, Surveillance

中图分类号: