中国媒介生物学及控制杂志 ›› 2022, Vol. 33 ›› Issue (5): 642-647.DOI: 10.11853/j.issn.1003.8280.2022.05.006

• 蜱类研究专题 • 上一篇    下一篇

内蒙古阿尔山地区游离蜱携带伯氏疏螺旋体的检测及基因分型研究

段立科1,2, 侯学霞2, 张琳2, 包子豪2, 刘泽梁1, 施淇源1, 周海健2, 董爱英1, 郝琴2   

  1. 1. 华北理工大学附属医院临床微生物科, 河北唐山 063000;
    2. 中国疾病预防控制中心传染病预防控制所螺旋体病控制室, 传染病预防控制国家重点实验室, 北京 102206
  • 收稿日期:2022-05-19 出版日期:2022-10-20 发布日期:2022-10-14
  • 通讯作者: 董爱英,E-mail:dongaiying68@126.com;郝琴,E-mail:haoqin@icdc.cn
  • 作者简介:段立科,女,在读硕士,主要从事蜱媒病原体检测工作,E-mail:isduanlike@163.com
  • 基金资助:
    国家科技重大专项(2017ZX10303404-006-003,2018ZX10101002-002)

Detection and genotyping of Borrelia burgdorferi carried by free-living ticks in Arxan,Inner Mongolia,China

DUAN Li-ke1,2, HOU Xue-xia2, ZHANG Lin2, BAO Zi-hao2, LIU Ze-liang1, SHI Qi-yuan1, ZHOU Hai-jian2, DONG Ai-ying1, HAO Qin2   

  1. 1. Clinical Microbiology Department, North China University of Science and Technology, Tangshan, Hebei 063000, China;
    2. State Key Laboratory of Infectious Disease Prevention and Control, Department of Spirochetosis Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2022-05-19 Online:2022-10-20 Published:2022-10-14
  • Supported by:
    National Science and Technology Major Project of China (No.2017ZX10303404-006-003,2018ZX10101002-002)

摘要: 目的 了解内蒙古阿尔山地区游离蜱的伯氏疏螺旋体感染状况及携带的基因型,为当地莱姆病的防控提供依据。方法 2020年5月和2021年5月,在内蒙古阿尔山地区用布旗法采集游离蜱,经形态学和特异性16S rDNA基因检测鉴定蜱种。随机选择113只蜱提取基因组DNA,采用巢式PCR扩增伯氏疏螺旋体5S~23S rRNA基因间隔区,阳性产物测序分析。采用BSK Ⅱ培养基对102只全沟硬蜱中携带的伯氏疏螺旋体进行分离培养,分离出的菌株采用多位点序列分析法(MLSA)鉴定基因型。结果 共采获游离蜱295只,经鉴定主要为全沟硬蜱(282/295,95.59%),其次为森林革蜱(13/295,4.41%)。113只蜱的PCR检测结果显示,100只全沟硬蜱中24只携带伯氏疏螺旋体,感染率为24.00%,其中17只感染Borrelia garinii基因型(17/24,70.83%),7只感染B. afzelii基因型(7/24,29.17%),13只森林革蜱中未检出伯氏疏螺旋体。对102只全沟硬蜱中携带的伯氏疏螺旋体进行分离培养,得到2株伯氏疏螺旋体菌株,经MLSA分型鉴定为B. garinii基因型。结论 内蒙古阿尔山地区5月游离蜱以全沟硬蜱为主,其中伯氏疏螺旋体感染率为24.00%,感染的基因型为B. gariniiB. afzelii 2种致病基因型,并分离出2株B. garinii菌株,证实内蒙古阿尔山地区存在伯氏疏螺旋体的自然感染。当地应关注伯氏疏螺旋体的感染和致病风险,开展人群、媒介蜱和宿主的监测工作,建立科学合理的莱姆病防控体系。

关键词: 莱姆病, 蜱, 伯氏疏螺旋体, 多位点序列分析

Abstract: Objective To understand the infection and genotypes of Borrelia burgdorferi carried by free-living ticks in Arxan,Inner Mongolia,China,and to provide a basis for local prevention and control of Lyme disease.Methods From May 2020 to May 2021,the drag-flag method was used to collect free-living ticks in Arxan.Tick species were identified by morphology and specific 16S rDNA gene sequencing.Genomic DNA was extracted from 113 randomly selected ticks.Nested-PCR targeting the 5S-23S rRNA intergenic spacer region was used to detect B.burgdorferi,and sequence analysis was performed to determine the genotypes of B.burgdorferi in ticks.The B.burgdorferi carried by 102 Ixodes persulcatus were isolated and cultured in BSK II medium.Multilocus sequence analysis (MLSA) was used to genotype the isolates.Results A total of 295 free-living ticks were collected,which were dominated by I. persulcatus(282/295,95.59%),followed by Dermacentor silvarum(13/295,4.41%).PCR detection of 113 ticks showed that 24 of 100 I. persulcatus carried B. burgdorferi,and the infection rate was 24.00%.Of these,17 were infected with B.garinii(17/24,70.83%),and 7 were infected with B.afzelii (7/24,29.17%).B.burgdorferi was not detected in 13 D.silvarum.Two strains of B. burgdorferi were isolated from the 102 I. persulcatus,which were identified as B. garinii through MLSA.Conclusion The free-living ticks in Arxan in May were dominated by I. persulcatus.The infection rate of B. burgdorferi in I. persulcatus was 24.00%,and the infection was caused by B. garinii and B. afzelii.Two B. garinii strains were isolated from ticks,which suggests that natural infection of spirochetes of Lyme disease was present in Arxan of Inner Mongolia.Local government should pay attention to the risk of B. burgdorferi infection as a causative agent of Lyme disease,monitor the populations,vector ticks,and hosts,and establish a system for the scientific prevention and control of Lyme disease.

Key words: Lyme disease, Tick, Borrelia burgdorferi, Multilocus sequence analysis

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