吉尔吉斯共和国进口羊毛携带蜱的监测与鉴定

doi:10.11853/j.issn.1003.8280.2020.01.009

中国媒介生物学及控制杂志 ›› 2020, Vol. 31 ›› Issue (1): 41-45.DOI: 10.11853/j.issn.1003.8280.2020.01.009

吉尔吉斯共和国进口羊毛携带蜱的监测与鉴定

王科珂¹, 何宇平², 王强², 林颖峥², 陈瑞花¹, 王艳², 史茜¹, 邓耀华², 徐新峰¹, 陈志飞², 李树清²

1 新疆出入境检验检疫局检验检疫技术中心动植物检测实验室, 新疆乌鲁木齐 830063;
2 上海出入境检验检疫局动植物与食品检验检疫技术中心, 上海 200135

收稿日期:2019-10-19 出版日期:2020-02-20 发布日期:2020-02-20
通讯作者: 李树清,Email:1323682242@qq.com
作者简介:王科珂,女,硕士,主要从事进出境动物疫病检验检疫,Email:ar_circle@163.com
基金资助:
国家重点研发计划（2016YFC1202000，2016YFC1202004）；海关总署科研计划项目（2018IK063）

Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China

WANG Ke-ke¹, HE Yu-ping², WANG Qiang², LIN Ying-zheng², CHEN Rui-hua¹, WANG Yan², SHI Qian¹, DENG Yao-hua², XU Xin-feng¹, CHEN Zhi-fei², LI Shu-qing²

1 Animal and Plant Test Laboratory, Inspection and Quarantine Technical Center of Xinjiang Entry-Exit Inspection and Quarantine Bureau, Urumqi 830063, Xinjiang Uygur Autonomous Region, China;
2 Technical Center for Animal, Plant and Food Inspection and Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau

Received:2019-10-19 Online:2020-02-20 Published:2020-02-20
Supported by:
Supported by the National Key R&D Program of China (No. 2016YFC1202000, 2016YFC1202004) and General Administration of Customs Research Project (No. 2018IK063)

摘要/Abstract

摘要： 目的为防止有害媒介生物通过国际贸易传入我国，对进口羊毛进行蜱监测与鉴定。方法采集2017年9月至2018年6月新疆维吾尔自治区吐尔尕特口岸从吉尔吉斯共和国进口的16批次羊毛，对其进行蜱检疫。将检出的蜱进行形态学鉴定，通过提取其基因组DNA，扩增线粒体16S rRNA基因、18S rRNA基因以及细胞色素C氧化酶亚基Ⅰ（COⅠ）基因并测序，利用MEGA 7.0软件构建系统发育树对其进行分子生物学鉴定。结果从吉尔吉斯共和国进口的16批次羊毛中有11批次检出蜱。共检出蜱39只，其中活体1只。经形态学和分子生物学鉴定，隶属2科4属5种，分别为拉合钝缘蜱、亚洲璃眼蜱、边缘革蜱、刻点血蜱和具沟血蜱。其中具沟血蜱30只，占截获蜱总数的76.9%，为吉尔吉斯共和国进口羊毛携带蜱的优势种类；活体蜱为拉合钝缘蜱若蜱。结论吉尔吉斯共和国进口羊毛携带蜱的概率较高，以具沟血蜱为主，且首次从进口羊毛中检出拉合钝缘蜱若蜱活体。因此，从吉尔吉斯共和国进口羊毛要加强对蜱的检疫，防止病媒生物入侵，严防疫病传入。

关键词: 吉尔吉斯共和国, 羊毛, 拉合钝缘蜱, 具沟血蜱, 鉴定

Abstract: Objective To monitor and identify the ticks carried by wool imported from Kyrgyz Republic to China, and to prevent the transmission of harmful vectors through international trade. Methods Sixteen batches of wool imported from Kyrgyz Republic at Turgart port, Xinjiang Uygur Autonomous Region, from September 2017 to June 2018 were examined to detect ticks. The detected ticks were subjected to morphological identification. Genomic DNA was extracted from the ticks, and the mitochondrial 16S rRNA gene, 18S rRNA gene, and cytochrome C oxidase subunitⅠgene were amplified and sequenced to construct a phylogenetic tree using MEGA 7.0 software for molecular biological identification. Results Sixteen batches of wool were imported from Kyrgyz Republic, and ticks were detected in 11 batches of the imported wool. A total of 39 ticks were detected, including 1 live tick. They belonged to 5 species of 4 genera in 2 families as identified by morphological and molecular phylogenetic analysis:Ornithodoros lahorensis, Hyalomma asiaticum, Dermacentor marginatus, Haemaphysalis punctata, and H. sulcata. There were 30 ticks of H. sulcata, as the predominant species (76.9% of the total number) of intercepted ticks carried by the wool imported from Kyrgyz Republic. The live tick was a nymph of O. lahorensis. Conclusion The intercepted ticks are probably detected in wool imported from Kyrgyz Republic, which are mainly H. sulcata. The live nymph of O. lahorensis was intercepted for the first time. Therefore, the quarantine of ticks carried by wool imported from Kyrgyz Republic should be strengthened to prevent vector invasion and transmission of harmful diseases.

Key words: Kyrgyz Republic, Wool, Ornithodoros lahorensis, Haemaphysalis sulcata, Identification

中图分类号:

R384.4

王科珂, 何宇平, 王强, 林颖峥, 陈瑞花, 王艳, 史茜, 邓耀华, 徐新峰, 陈志飞, 李树清. 吉尔吉斯共和国进口羊毛携带蜱的监测与鉴定[J]. 中国媒介生物学及控制杂志, 2020, 31(1): 41-45.

WANG Ke-ke, HE Yu-ping, WANG Qiang, LIN Ying-zheng, CHEN Rui-hua, WANG Yan, SHI Qian, DENG Yao-hua, XU Xin-feng, CHEN Zhi-fei, LI Shu-qing. Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China[J]. Chines Journal of Vector Biology and Control, 2020, 31(1): 41-45.

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吉尔吉斯共和国进口羊毛携带蜱的监测与鉴定

Surveillance and identification of intercepted ticks carried by wool imported from Kyrgyz Republic to Xinjiang Uygur Autonomous Region, China

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