中国媒介生物学及控制杂志 ›› 2019, Vol. 30 ›› Issue (1): 78-79.DOI: 10.11853/j.issn.1003.8280.2019.01.018

• 调查研究 • 上一篇    下一篇

长角血蜱野外种群对常用杀虫剂相对敏感基线和诊断剂量研究

赵奇, 高丽君, 张玉勤, 刘吉起   

  1. 河南省疾病预防控制中心消毒与媒介生物控制室, 河南 郑州 450016
  • 收稿日期:2018-09-10 出版日期:2019-02-20 发布日期:2019-02-20
  • 通讯作者: 刘吉起,Email:hncdcljq@sina.com
  • 作者简介:赵奇,男,硕士,主要从事媒介生物控制研究工作,Email:zhaoqi23@foxmail.com
  • 基金资助:
    河南省医学科技攻关计划项目(2011020176)

A study on the relative susceptibility baselines and diagnostic doses of wild population of Haemaphysalis longicornis to common insecticides

ZHAO Qi, GAO Li-jun, ZHANG Yu-qin, LIU Ji-qi   

  1. Henan Center for Disease Control and Prevention, Zhengzhou 450016, Henan Province, China
  • Received:2018-09-10 Online:2019-02-20 Published:2019-02-20
  • Supported by:
    Supported by the Medical Science and Technique Project of Henan Province (No. 2011020176)

摘要: 目的 建立长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量。方法 分别于2013年和2014年4-9月采集河南省王屋山区未接触过任何杀虫剂的长角血蜱野外种群。采用药膜法测定长角血蜱成蜱对杀虫剂的敏感性。采用DPS数据处理软件进行机值分析,拟合毒力回归方程,确定半数致死剂量、99%致死剂量,计算诊断剂量。结果 建立了对敌敌畏、双硫磷、仲丁威、氯菊酯、溴氰菊酯和高效氯氰菊酯6种杀虫剂的相对敏感基线,分别为y=1.549 9+1.359 4xy=2.343 3+1.225 6xy=-6.850 7+5.213 9xy=0.866 1+1.571 1xy=0.411 6+2.277 8xy=2.551 8+1.018 9x;相应的诊断剂量分别为35.505 6、10.764 7、1.047 4、25.872 9、2.171 4和97.063 2 mg/L。结论 建立了长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量,可作为长角血蜱抗药性监测的参考。

关键词: 长角血蜱, 诊断剂量, 抗药性, 抗药性监测

Abstract: Objective To establish the relative susceptibility baselines and diagnostic doses of the wild population of Haemaphysalis longicornis to common insecticides. Methods The wild population of H. longicornis without exposure to any pesticides was collected at Mount Wangwu in Henan, China from April to September in both 2013 and 2014. The residual film method was used to determine the susceptibility of adult H. longicornis to insecticides. DPS data processing software was used for probit analysis, and the toxicity regression equation was fitted to determine the median lethal dose and 99% lethal dose and then to calculate the diagnostic dose. Results The susceptibility baselines of H. longicornis to dichlorvos, temephos, bassa, permethrin, deltamethrin, and beta-cypermethrin were y=1.549 9+1.359 4x, y=2.343 3+1.225 6x, y=-6.850 7+5.213 9x, y=0.866 1+1.571 1x, y=0.411 6+2.277 8x, and y=2.551 8+1.018 9x, respectively; the diagnostic doses were 35.505 6, 10.764 7, 1.047 4, 25.872 9, 2.171 4, and 97.063 2 mg/L, respectively. Conclusion The relative susceptibility baselines of the wild population of H. longicornis to the six insecticides, as well as the diagnostic doses, have been established. The results can be used as a reference for monitoring the resistance of H. longicornis to pesticides.

Key words: Haemaphysalis longicornis, Diagnostic dose, Insecticide resistance, Resistance monitoring

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