Application of DNA barcoding in species identification of bedbugs
ZHANG Rui-ling1,2, HUANG Zhen-dong1, LIU Tong-kai1, ZHAO Ai-hua3, ZHANG Zhong1,2
1 Department of Pathogenic Biology of Taishan Medical University, Tai'an 271016, Shandong Province, China; 2 Collaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Taishan Medical University; 3 Tai′an Center for Disease Control and Prevention
Abstract：Objective To make accurate identification of bedbug species and provide information for implementation of effective prevention and control measures. Methods In this study, morphological identification and DNA barcoding that distinguishes species based on the differential of genetic distances of mitochondrial Cytochrome C oxidase subunit Ⅰgene (COⅠ), were employed to identify the bedbugs collected from a school of Tai'an in Shandong province. Results Firstly, length of pronotum is two times of width, corresponding with the description of Cimex lectularius. Secondly, genetic distances of unidentified bedbug samples with C. lectularius were 0-1.3%, while 27.6%-28.0% with C. hemipterus. Conclusion Both morphological characters and genetic distances supported that these bedbug samples belong to C. lectularius. Bedbugs are social insects, which is usually spread by human activities. Implementation of long term effective prevention and control measures are crucial for reducing and avoiding the threat caused by bedbugs.
张瑞玲, 黄振东, 刘同凯, 赵爱华, 张忠. DNA条形码技术在臭虫物种鉴定中的应用[J]. 中国媒介生物学及控制杂志, 2018, 29(5): 458-461.
ZHANG Rui-ling, HUANG Zhen-dong, LIU Tong-kai, ZHAO Ai-hua, ZHANG Zhong. Application of DNA barcoding in species identification of bedbugs. Chines Journal of Vector Biology and Control, 2018, 29(5): 458-461.
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2018, Vol. 29 
