Identification of Berylmys bowersi based on DNA barcoding in Guangdong province
YAO Dan-dan, FENG Zhi-yong, JIANG Hong-xue
Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, Guangdong Province, China
Abstract：Objective Two previously unknown rodent species were identified by DNA barcoding from Yangchun city, Guangdong province. Methods Genomic DNA was extracted from samples, and the mitochondrial cytochrome c oxidase subunitⅠ genes were amplified using universal primers (BatL5310 and R6036R), then sequenced. BLAST comparison was subsequently carried out in NCBI. The phylogenetic tree was constructed using the Neighbor-Joining method. Genetic distances were calculated based on double parameter model. Results DNA sequences from two samples were consistent, suggesting they were the same species. It showed high sequence similarities with Berylmys bowersi(99%) in GenBank. In addition, genetic distance with B. bowersi was 0.004 6, less than 1% threshold of intraspecific genetic distance. Therefore, the unknown species was B. bowersi. Conclusion DNA barcoding is a highly effective approach to rapidly identify species, which could make up the shortages of morphological taxonomy.
姚丹丹, 冯志勇, 姜洪雪. 广东省青毛鼠的DNA条形码鉴定[J]. 中国媒介生物学及控制杂志, 2018, 29(2): 143-146.
YAO Dan-dan, FENG Zhi-yong, JIANG Hong-xue. Identification of Berylmys bowersi based on DNA barcoding in Guangdong province. Chines Journal of Vector Biology and Control, 2018, 29(2): 143-146.
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2018, Vol. 29 
